Competitive CatSper Activators of Progesterone from Rhynchosia volubilis
文献类型：期刊论文
| 作者 | Xiang, Jin1; Kang, Hang2,3; Li, Hong-Gang4; Shi, Yu-Long6,7; Zhang, Ya-Li5; Ruan, Chang-Lei1; Liu, Lin-Hui1; Gao, Han-Qi1; Luo, Tao2,3; Hu, Gao-Sheng5 |
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2021-08-06 |
| 页码 | 10 |
| 关键词 | Leguminosae Rhynchosia volubilis CatSper prenylated isoflavonoids rhynchones A - E |
| ISSN号 | 0032-0943 |
| DOI | 10.1055/a-1542-0151 |
| 通讯作者 | Fang, Jin-Bo(fangjb@hust.edu.cn) |
| 英文摘要 | The root Rhynchosia volubilis was widely used for contraception in folk medicine, although its molecular mechanism on antifertility has not yet been revealed. In human sperm, it was reported that the cation channel of sperm, an indispensable cation channel for the fertilization process, could be regulated by various steroid-like compounds in plants. Interestingly, these nonphysiological ligands would also disturb the activation of the cation channel of sperm induced by progesterone. Therefore, this study aimed to explore whether the compounds in R. volubilis affect the physiological regulation of the cation channel of sperm. The bioguided isolation of the whole herb of R. volubilis has resulted in the novel discovery of five new prenylated isoflavonoids, rhynchones A-E ( 1 - 5 ), a new natural product, 5 '- O -methylphaseolinisoflavan ( 6 ) ( (1) H and (13) C NMR data, Supporting Information), together with twelve known compounds ( 7 - 18 ). Their structures were established by extensive spectroscopic analyses and drawing a comparison with literature data, while their absolute configurations were determined by electronic circular dichroism calculations. The experiments of intracellular Ca (2+) signals and patch clamping recordings showed that rhynchone A ( 1 ) significantly reduced cation channel of sperm activation by competing with progesterone. In conclusion, our findings indicat that rhynchone A might act as a contraceptive compound by impairing the activation of the cation channel of sperm and thus prevent fertilization. |
| WOS关键词 | CHEMICAL-CONSTITUENTS ; CIRCULAR-DICHROISM ; ISOFLAVONOIDS ; EXTRACT ; CHANNEL ; SPERM |
| 资助项目 | National Key Research and Development Plan[2016YFC1000905] ; National Natural Science Foundation[31000150] ; Fundamental Research Funds for the Central Universities[HUST2016YXMS145] ; Special Funds for Central Government to Guide Local Scientific and Technological Development[20202ZDB01013] ; Open Funds of State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics[T152602] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000682039900004 |
| 出版者 | GEORG THIEME VERLAG KG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298119]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Fang, Jin-Bo |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Hangkong Rd 13, Wuhan 430030, Hubei, Peoples R China 2.Nanchang Univ, Key Lab Reprod Physiol & Pathol Jiangxi Prov, Inst Life Sci, Nanchang, Jiangxi, Peoples R China 3.Nanchang Univ, Key Lab Reprod Physiol & Pathol Jiangxi Prov, Sch Life Sci, Nanchang, Jiangxi, Peoples R China 4.Huazhong Univ Sci & Technol, Tongji Med Coll, Inst Reprod Hlth, Ctr Reprod Med, Wuhan, Peoples R China 5.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang, Peoples R China 6.Chinese Acad Sci, CAS Key Lab Receptor Res & Drug Discovery & Desig, Shanghai Inst Mat Med, Shanghai, Peoples R China 7.Univ Chinese Acad Sci, Sch Pharm, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiang, Jin,Kang, Hang,Li, Hong-Gang,et al. Competitive CatSper Activators of Progesterone from Rhynchosia volubilis[J]. PLANTA MEDICA,2021:10. |
| APA | Xiang, Jin.,Kang, Hang.,Li, Hong-Gang.,Shi, Yu-Long.,Zhang, Ya-Li.,...&Fang, Jin-Bo.(2021).Competitive CatSper Activators of Progesterone from Rhynchosia volubilis.PLANTA MEDICA,10. |
| MLA | Xiang, Jin,et al."Competitive CatSper Activators of Progesterone from Rhynchosia volubilis".PLANTA MEDICA (2021):10. |
入库方式： OAI收割
来源：上海药物研究所
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