UPLC-ESI-Q-TOF/MS Based Metabolic Profiling of Protosappanin B in Rat Plasma, Bile, Feces, Urine and Intestinal Bacteria Samples
文献类型：期刊论文
| 作者 | Chen, Wei-Ying3,6; Liang, Guang-Yan1; Zheng, Zuo-Liang2,3,6; Wu, Yun-Shan2,3,6; Xu, Fang-Fang2,3,6; Liu, Bo2,3,5,6; Guo, De-an3,4,6
|
| 刊名 | CURRENT DRUG METABOLISM
 |
| 出版日期 | 2021 |
| 卷号 | 22期号:6页码:491-499 |
| ISSN号 | 1389-2002 |
| 关键词 | Protosappanin B Metabolic pathways UPLC-TOF-MS MS Plasma Bile Feces Urine Intestinal bacteria sample |
| DOI | 10.2174/1389200222666210219123846 |
| 通讯作者 | Liu, Bo(doctliu@263.net) ; Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | Background: Caesalpinia sappan L. is a traditional medicinal plant that is used to promote blood circulation and treat stroke in China. Protosappanin B (PTB) is a unique homoisoflavone compound isolated from Sappan Lignum (the heartwood of Caesalpinia sappan L). In a previous study, the metabolic fate of PTB remained unknown. Objective: To explore whether PTB is extensively metabolized, the metabolites of PTB in bile, plasma, urine, feces, and intestinal bacteria samples in rats were investigated. Methods: The biosamples were investigated by ultraperformance liquid chromatography combined with time-offlight mass spectrometry (UPLC-TOF-MS/MS) with MetabolitePilot software. Results: 28 metabolites were identified in the biosamples: 18 metabolites in rat bile, 8 in plasma, 20 in feces, 7 in urine and 2 in intestinal bacteria samples. Both phase I and phase II metabolites were observed. Metabolite conversion occurred via 9 proposed pathways: sulfate conjugation, glucuronide conjugation, bis-glucuronide conjugation, glucose conjugation, dehydration, oxidation, hydrolysis, methylation and hydroxymethylene loss. The metabolic pathways differed among biosamples and exhibited different distributions. Among these pathways, the most important was sulfate and glucuronide conjugation. Conclusion: The results showed that the small intestinal and biliary routes play an important role in the clearance and excretion of PTB. The main sites of metabolism in the PTB chemical structure were the phenolic hydroxyl and the side-chains on the eight-element ring. |
| WOS关键词 | SAPPAN LIGNUM ; IN-VITRO ; CONSTITUENTS ; BRAZILIN |
| 资助项目 | Research Project of Traditional Chinese Medicine Bureau of Guangdong Province[20211186] ; National Key Research and Development Program of China[2018YFC1707900] ; National Natural Science Foundation of China[81202398] ; National Natural Science Foundation of China[81703379] ; special foundation of Guangzhou Key Laboratory[202002010004] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | BENTHAM SCIENCE PUBL LTD |
| WOS记录号 | WOS:000676363600007 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298268]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Bo; Guo, De-an |
| 作者单位 | 1.Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Peoples R China 2.Guangzhou Key Lab Chiral Res Act Components Tradi, Guangzhou 510006, Peoples R China 3.Guangzhou Univ Chinese Med, Clin Med Coll 2, Guangzhou 510006, Guangdong, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China 5.Guangzhou Univ Chinese Med, Guangdong Hong Kong Macau Joint Lab Chinese Med &, Guangzhou 510006, Peoples R China 6.Guangzhou Univ Chinese Med, Guangdong Prov Key Lab Chinese Med Prevent & Trea, Guangzhou 510006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Wei-Ying,Liang, Guang-Yan,Zheng, Zuo-Liang,et al. UPLC-ESI-Q-TOF/MS Based Metabolic Profiling of Protosappanin B in Rat Plasma, Bile, Feces, Urine and Intestinal Bacteria Samples[J]. CURRENT DRUG METABOLISM,2021,22(6):491-499. |
| APA | Chen, Wei-Ying.,Liang, Guang-Yan.,Zheng, Zuo-Liang.,Wu, Yun-Shan.,Xu, Fang-Fang.,...&Guo, De-an.(2021).UPLC-ESI-Q-TOF/MS Based Metabolic Profiling of Protosappanin B in Rat Plasma, Bile, Feces, Urine and Intestinal Bacteria Samples.CURRENT DRUG METABOLISM,22(6),491-499. |
| MLA | Chen, Wei-Ying,et al."UPLC-ESI-Q-TOF/MS Based Metabolic Profiling of Protosappanin B in Rat Plasma, Bile, Feces, Urine and Intestinal Bacteria Samples".CURRENT DRUG METABOLISM 22.6(2021):491-499. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。