Acyl-CoA oxidase ACOX-1 interacts with a peroxin PEX-5 to play roles in larval development of Haemonchus contortus
文献类型：期刊论文
| 作者 | Shi, Hengzhi2; Huang, Xiaocui3; Chen, Xueqiu2; Yang, Yi2; Wang, Zhao2; Yang, Yimin2; Wu, Fei2; Zhou, Jingru2; Yao, Chaoqun1,4; Ma, Guangxu2,5 |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2021-07-01 |
| 卷号 | 17期号:7页码:21 |
| ISSN号 | 1553-7366 |
| DOI | 10.1371/journal.ppat.1009767 |
| 通讯作者 | Ma, Guangxu(gxma1@zju.edu.cn) ; Du, Aifang(afdu@zju.edu.cn) |
| 英文摘要 | Hypobiosis (facultative developmental arrest) is the most important life-cycle adaptation ensuring survival of parasitic nematodes under adverse conditions. Little is known about such survival mechanisms, although ascarosides (ascarylose with fatty acid-derived side chains) have been reported to mediate the formation of dauer larvae in the free-living nematode Caenorhabditis elegans. Here, we investigated the role of a key gene acox-1, in the larval development of Haemonchus contortus, one of the most important parasitic nematodes that employ hypobiosis as a routine survival mechanism. In this parasite, acox-1 encodes three proteins (ACOXs) that all show a fatty acid oxidation activity in vitro and in vivo, and interact with a peroxin PEX-5 in peroxisomes. In particular, a peroxisomal targeting signal type1 (PTS1) sequence is required for ACOX-1 to be recognised by PEX-5. Analyses on developmental transcription and tissue expression show that acox-1 is predominantly expressed in the intestine and hypodermis of H. contortus, particularly in the early larval stages in the environment and the arrested fourth larval stage within host animals. Knockdown of acox-1 and pex-5 in parasitic H. contortus shows that these genes play essential roles in the post-embryonic larval development and likely in the facultative arrest of this species. A comprehensive understanding of these genes and the associated beta-oxidation cycle of fatty acids should provide novel insights into the developmental regulation of parasitic nematodes, and into the discovery of novel interventions for species of socioeconomic importance. Author summary Haemonchus contortus is one of the most pathogenic nematodes causing substantial economic losses worldwide that undergo facultative arrest (diapause) to survive harsh environmental and host conditions. However, the molecular basis (e.g., fatty acid oxidation and dauer pheromone synthesis) underlying such survival mechanisms has not yet been elucidated in parasitic nematodes. Here, we show that an acyl-CoA oxidase ACOX-1 interacts with a peroxin PEX-5 and functions in fatty acid oxidation in peroxisomes. Further, knockdown of ACOX-1 and PEX-5 results in a lethal phenotype of the infective stage and likely developmental parasitic larval stage within host animals. This data demonstrates an essential role of ACOX-1-PEX-5 and potentially ascarosides signalling in the facultative arrested larval development of parasitic nematodes, and suggests possible intervention targets. |
| WOS关键词 | SMALL-MOLECULE SIGNALS ; CAENORHABDITIS-ELEGANS ; SACCHAROMYCES-CEREVISIAE ; DAUER PHEROMONE ; ARRESTED DEVELOPMENT ; RECEPTOR ; BIOGENESIS ; DIAPAUSE ; PROTEIN ; GENE |
| 资助项目 | National Key Basic Research Program of China[2015CB150300] ; National Key Research and Development Program of China[2017YFD0501200] ; National Natural Science Foundation of China[32002304] ; State Key Laboratory of Pathogen Biology of Livestock Diseases Open Foundation[SKLVEB2016KFKT018] |
| WOS研究方向 | Microbiology ; Parasitology ; Virology |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000677723400002 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298283]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ma, Guangxu; Du, Aifang |
| 作者单位 | 1.Ross Univ, Sch Vet Med, Hlth Ctr Zoonoses & Trop Vet Med 1, Basseterre, St Kitts & Nevi 2.Zhejiang Univ, Coll Anim Sci, Inst Prevent Vet Med, Zhejiang Prov Key Lab Prevent Vet Med, Hangzhou, Zhejiang, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 4.Ross Univ, Sch Vet Med, Dept Biomed Sci, Basseterre, St Kitts & Nevi 5.Univ Melbourne, Melbourne Vet Sch, Dept Vet Biosci, Parkville, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Shi, Hengzhi,Huang, Xiaocui,Chen, Xueqiu,et al. Acyl-CoA oxidase ACOX-1 interacts with a peroxin PEX-5 to play roles in larval development of Haemonchus contortus[J]. PLOS PATHOGENS,2021,17(7):21. |
| APA | Shi, Hengzhi.,Huang, Xiaocui.,Chen, Xueqiu.,Yang, Yi.,Wang, Zhao.,...&Du, Aifang.(2021).Acyl-CoA oxidase ACOX-1 interacts with a peroxin PEX-5 to play roles in larval development of Haemonchus contortus.PLOS PATHOGENS,17(7),21. |
| MLA | Shi, Hengzhi,et al."Acyl-CoA oxidase ACOX-1 interacts with a peroxin PEX-5 to play roles in larval development of Haemonchus contortus".PLOS PATHOGENS 17.7(2021):21. |
入库方式： OAI收割
来源：上海药物研究所
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