The mid-Proterozoic (ca. 1.85-0.85 Ga) might have been environmentally "boring", but was a crucial interval of time for the early establishment of eukaryotic life on Earth. The Mesoproterozoic shale-dominated Xiamaling Formation (ca. 1.4-1.35 Ga) in North China preserved abundant organic-walled microfossils, some of which were of eukaryotic affinity but their taxonomic diversity and evolutionary implications remain unclear. In this paper, we present a palynological study of the lower Xiamaling Formation in the northern Tianjin Municipality. Similar to other Mesoproterozoic microbiotas, this assemblage consists of well-preserved and diverse spheroidal to ellipsoidal vesicles, filamentous microfossils and cellular aggregates. In total, 36 species belonging to 28 genera are identified, including 1 new species Quadrimurus clavatus gen. et sp. nov., and 5 unnamed forms. Majority of these taxa are recognised for the first time from this formation, indicating a richer assemblage than previously known. Although most taxa are of unknown biological affinities, this microbiota is thought to be composed of prokaryotic and eukaryotic microorganisms, in which 12 taxa are inferred to be eukaryotic. These microfossils indicate a moderate diversity of eukaryotic life documented in the Xiamaling Formation, which is consistent with other microbiotas in the mid-Proterozoic. The presence of Jixiania lineata Yan, 1986 (=Lineaforma elongata Vorob'eva and Sergeev, 2015) in North China extends its geographic distribution and further enhances its potential as index fossil for the early Mesoproterozoic strata. The Xiamaling assemblage provides one of the broader pictures of the Mesoproterozoic biosphere, in which eukaryotes were predominantly protistan-grade microorganisms.