A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol
文献类型：期刊论文
| 作者 | Liu, G ; Chen, HD ; Peng, HZ（彭红珍） ; Song, SP（宋世平） ; Gao, JM ; Lu, JX ; Ding, M ; Li, LY ; Ren, SZ ; Zou, ZY ; Fan, CH（樊春海） |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2011 |
| 卷号 | 28期号:1页码:308 |
| ISSN号 | 0956-5663 |
| 英文摘要 | Carbon nanotubes have shown their unique advantages of mechanical, chemical and electronic properties in bioanalysis. We herein report a new method to efficiently and reproducibly prepare multi-walled carbon nanotubes (MWNTs)-protein sensing layers for electrochemical immunosensors. This method employs centrifugation to prepare a conjugate of MWNTs and goat anti mouse-immunoglobulin G (IgG) (secondary antibody). The conjugates were then deposited on screen-printed electrodes to form a nanostructured layer (MWNT-I layer). CLB monoclonal antibody was assembled through its binding to the secondary antibody. The MWNT-I layer-based electrodes were used for rapid and sensitive amperometric immunosensing detection of clenbuterol (CLB) in swine urine samples. Horseradish peroxidase-coupled CLB (CLB-HRP) competed with free CLB in the samples to bind the monoclonal antibody. It has shown significantly higher sensitivity and better reproducibility than the chemical conjugation method. This MWNT-based immunosensor is highly sensitive, leading to a limit of detection of 0.1 ng/mL within a rapid assay time of 16 min. Its sensitivity is at least 1 order of magnitude higher than that of a normal immunosensor (without MWNTs). The sensing device is portable with disposable screen-printed electrode, satisfactorily meeting the requirements for field detection of food security-related species. (C) 2011 Elsevier B.V. All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295661700047 |
| 公开日期 | 2013-09-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/12843]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Liu, G,Chen, HD,Peng, HZ,et al. A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol[J]. BIOSENSORS & BIOELECTRONICS,2011,28(1):308. |
| APA | Liu, G.,Chen, HD.,Peng, HZ.,Song, SP.,Gao, JM.,...&Fan, CH.(2011).A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol.BIOSENSORS & BIOELECTRONICS,28(1),308. |
| MLA | Liu, G,et al."A carbon nanotube-based high-sensitivity electrochemical immunosensor for rapid and portable detection of clenbuterol".BIOSENSORS & BIOELECTRONICS 28.1(2011):308. |
入库方式： OAI收割
来源：上海应用物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。