A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I
文献类型：期刊论文
| 作者 | Xu, H ; Gao, SL ; Liu, QW ; Pan, D（潘敦） ; Wang, LH(王丽华） ; Ren, SZ ; Ding, M ; Chen, JW ; Liu, G |
| 刊名 | SENSORS
 |
| 出版日期 | 2011 |
| 卷号 | 11期号:11页码:10187 |
| ISSN号 | 1424-8220 |
| 英文摘要 | We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297698600013 |
| 公开日期 | 2013-09-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/12846]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Xu, H,Gao, SL,Liu, QW,et al. A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I[J]. SENSORS,2011,11(11):10187. |
| APA | Xu, H.,Gao, SL.,Liu, QW.,Pan, D.,Wang, LH.,...&Liu, G.(2011).A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I.SENSORS,11(11),10187. |
| MLA | Xu, H,et al."A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I".SENSORS 11.11(2011):10187. |
入库方式： OAI收割
来源：上海应用物理研究所
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