Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein
文献类型：期刊论文
| 作者 | Zhang, QZ ; Zhao, B ; Yan, J（颜娟） ; Song, SP（宋世平） ; Min, R ; Fan, CH（樊春海） |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 83期号:23页码:9191 |
| ISSN号 | 0003-2700 |
| 英文摘要 | We have developed a nanotube-based colorimetric probe using multiwalled carbon nanotubes (MWNTs), anti-immunoglobulin G (anti-IgG), and horseradish peroxidase (HRP). The probe was used as an alternative to conventional colorimetric conjugates to obtain amplified signals in a sandwich-type immunoassay for ataxia telangiectasia mutated (ATM), a potential biomarker for radiation doses and cancers. Results show that the MWNT-based probe colorimetry was 5000 times more sensitive than a conventional ELISA, while its concentration range was 10 000 times wider than that of the latter. Its limit of detection (LOD) was 0.2, fg/mL (54 aM, similar to 32 molecules in 1 mu L samples). Control experiments showed that detection of ATM molecules at the picogram-level could still be achieved in samples that contained protein makers present at more than 100 times the ATM concentration, demonstrating the high specificity of the technique. The MWNT-based probe also has the potential to become a universal probe for colorimetric assays of most protein markers because it can recognize the associated rabbit polyclonal antibodies. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297481700054 |
| 公开日期 | 2013-09-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/12871]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhang, QZ,Zhao, B,Yan, J,et al. Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein[J]. ANALYTICAL CHEMISTRY,2011,83(23):9191. |
| APA | Zhang, QZ,Zhao, B,Yan, J,Song, SP,Min, R,&Fan, CH.(2011).Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein.ANALYTICAL CHEMISTRY,83(23),9191. |
| MLA | Zhang, QZ,et al."Nanotube-Based Colorimetric Probe for Ultrasensitive Detection of Ataxia Telangiectasia Mutated Protein".ANALYTICAL CHEMISTRY 83.23(2011):9191. |
入库方式： OAI收割
来源：上海应用物理研究所
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