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Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel
文献类型：期刊论文
| 作者 | Zhang, Xu ; Ren, Wenlin ; DeCaen, Paul ; Yan, Chuangye ; Tao, Xiao ; Tang, Lin（唐琳） ; Wang, Jingjing ; Hasegawa, Kazuya ; Kumasaka, Takashi ; He, Jianhua（何建华） ; Wang, Jiawei ; Clapham, David E. ; Yan, Nieng |
| 刊名 | NATURE
 |
| 出版日期 | 2012 |
| 卷号 | 486期号:7401页码:130 |
| ISSN号 | 0028-0836 |
| 英文摘要 | Voltage-gated sodium (Na-v) channels are essential for the rapid depolarization of nerve and muscle(1), and are important drug targets(2). Determination of the structures of Na-v channels will shed light on ion channel mechanisms and facilitate potential clinical applications. A family of bacterial Na-v channels, exemplified by the Na+-selective channel of bacteria (NaChBac)(3), provides a useful model system for structure-function analysis. Here we report the crystal structure of NavRh, a NaChBac orthologue from the marine alphaproteobacterium HIMB114 (Rickettsiales sp. HIMB114; denoted Rh), at 3.05 angstrom resolution. The channel comprises an asymmetric tetramer. The carbonyl oxygen atoms of Thr 178 and Leu 179 constitute an inner site within the selectivity filter where a hydrated Ca2+ resides in the crystal structure. The outer mouth of the Na+ selectivity filter, defined by Ser 181 and Glu 183, is closed, as is the activation gate at the intracellular side of the pore. The voltage sensors adopt a depolarized conformation in which all the gating charges are exposed to the extracellular environment. We propose that NavRh is in an 'inactivated' conformation. Comparison of NavRh with Na(v)Ab(4) reveals considerable conformational rearrangements that may underlie the electromechanical coupling mechanism of voltage-gated channels. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000304854000042 |
| 公开日期 | 2013-09-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/12895]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Zhang, Xu,Ren, Wenlin,DeCaen, Paul,et al. Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel[J]. NATURE,2012,486(7401):130. |
| APA | Zhang, Xu.,Ren, Wenlin.,DeCaen, Paul.,Yan, Chuangye.,Tao, Xiao.,...&Yan, Nieng.(2012).Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel.NATURE,486(7401),130. |
| MLA | Zhang, Xu,et al."Crystal structure of an orthologue of the NaChBac voltage-gated sodium channel".NATURE 486.7401(2012):130. |
入库方式： OAI收割
来源：上海应用物理研究所
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