Anti-VEGFR2-conjugated PLGA microspheres as an x-ray phase contrast agent for assessing the VEGFR2 expression
文献类型：期刊论文
| 作者 | Tang, RBA ; Chai, WM ; Ying, WH ; Yang, GY ; Xie, HL（谢红兰） ; Liu, HQ ; Chen, KM |
| 刊名 | PHYSICS IN MEDICINE AND BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 57期号:10 |
| ISSN号 | 0031-9155 |
| 英文摘要 | The primary goal of this study was to evaluate the feasibility of using anti-vascular endothelial growth factor receptor 2 (VEGFR2)-conjugated poly(lactic-co-glycolic acid) (PLGA) microspheres as an x-ray phase contrast agent to assess the VEGFR2 expression in cell cultures. The cell lines, mouse LLC (Lewis lung carcinoma) and HUVEC (human umbilical vein endothelial cell), were selected for cell adhesion studies. The bound PLGA microspheres were found to better adhere to LLC cells or HUVECs than unbound ones. Absorption and phase contrast images of PLGA microspheres were acquired and compared in vitro. Phase contrast imaging (PCI) greatly improves the detection of the microspheres as compared to absorption contrast imaging. The cells incubated with PLGA microspheres were imaged by PCI, which provided clear 3D visualization of the beads, indicating the feasibility of using PLGA microspheres as a contrast agent for phase contrast CT. In addition, the microspheres could be clearly distinguished from the wall of the vessel on phase contrast CT images. Therefore, the approach holds promise for assessing the VEGFR2 expression on endothelial cells of tumor-associated vessels. We conclude that PLGA microsphere-based PCI of the VEGFR2 expression might be a novel, promising biomarker for future studies of tumor angiogenesis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000303677900018 |
| 公开日期 | 2013-09-11 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/13092]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Tang, RBA,Chai, WM,Ying, WH,et al. Anti-VEGFR2-conjugated PLGA microspheres as an x-ray phase contrast agent for assessing the VEGFR2 expression[J]. PHYSICS IN MEDICINE AND BIOLOGY,2012,57(10). |
| APA | Tang, RBA.,Chai, WM.,Ying, WH.,Yang, GY.,Xie, HL.,...&Chen, KM.(2012).Anti-VEGFR2-conjugated PLGA microspheres as an x-ray phase contrast agent for assessing the VEGFR2 expression.PHYSICS IN MEDICINE AND BIOLOGY,57(10). |
| MLA | Tang, RBA,et al."Anti-VEGFR2-conjugated PLGA microspheres as an x-ray phase contrast agent for assessing the VEGFR2 expression".PHYSICS IN MEDICINE AND BIOLOGY 57.10(2012). |
入库方式： OAI收割
来源：上海应用物理研究所
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