Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead
文献类型：期刊论文
| 作者 | Zhu, Jingjing1,6; Zheng, Shuangshuang1; Liu, Hanbo6; Wang, Yiwei2; Jiao, Zeren4; Nie, Yi1,3; Wang, Hong5; Liu, Tianqing6; Song, Kedong6 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2021-03-31 |
| 卷号 | 174页码:339-351 |
| 关键词 | Crocin Anti-tumor Three-dimensional cell model |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2021.01.181 |
| 英文摘要 | Crocin, as one of the biologically active components of saffron, has anti-inflammatory, anti-oxidant, antidepressant and auxiliary anti-tumor effects. Studies have shown that crocin could promote breast cancer cell apoptosis. However, conventional methods are mainly based on two-dimensional (2D) cell culture models, which are difficult to reproduce the tumor environment in vivo due to space constraints. In this study, we prepared a three-dimensional (3D) cell model in vitro based on sodium alginate/gelatin to evaluate the inhibitory effect of crocin on MCF-7 cells, which could bridge the gap in crocin drug evaluation between 2D and 3D cell model in vitro. Different from the 2D culture, the cells were found to aggregate in a spherical shape in the 3D microgel beads. And the CCK-8 assay showed that the growth of MCF-7 cells exposed to crocin was inhibited in a time-related and concentration-related manner. Compared with 2D culture (IC50 that MCF-7 cells treated with crocin at 24 h, 48 h. 72 h: 3.68, 2.55 and 1.53 mg/mL, respectively), the IC50 value of 3D culture (IC50 that MCF-7 cells treated with crocin at 24 h, 48 h, 72 h: 10.12, 6.89 and 6.64 mg/mL, respectively) was significantly increased by 2.77, 2.70, 4.34 times, respectively. Besides, live/dead staining and scanning electron microscope (SEM) revealed that the 2D cultured cells shrank and ruptured after crocin treatment, and the number of living cells was considerably reduced: the size of the cell colonies in the 3D microgel beads decreased. (C) 2021 Published by Elsevier B.V. |
| 资助项目 | National Natural Science Foundation of China[31670978/21676041] ; Fok Ying Tung Education Foundation[132027] ; State Key Laboratory of Fine Chemicals[KF1111] ; Natural Science Foundation of Liaoning Province[20180510028] ; Fundamental Research Funds for the Central Universities[DUT20ZD206/DUT20YG118] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000625633200034 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; Fok Ying Tung Education Foundation ; State Key Laboratory of Fine Chemicals ; Natural Science Foundation of Liaoning Province ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48121]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Hong; Liu, Tianqing; Song, Kedong |
| 作者单位 | 1.Zhengzhou Inst Emerging Ind Technol, Zhengzhou 450000, Peoples R China 2.Univ Sydney, ANZAC Res Inst, Burns Res Grp, Concord, NSW 2139, Australia 3.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 4.Texas A&M Univ, Artie McFerrin Dept Chem Engn, College Stn, TX 77843 USA 5.Dalian Med Univ, Dept Spine Surg, Affiliated Hosp 1, Inst Canc Stem Cell, Dalian 116011, Peoples R China 6.Dalian Univ Technol, State Key Lab Fine Chem, Dalian R&D Ctr Stem Cell & Tissue Engn, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Jingjing,Zheng, Shuangshuang,Liu, Hanbo,et al. Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,174:339-351. |
| APA | Zhu, Jingjing.,Zheng, Shuangshuang.,Liu, Hanbo.,Wang, Yiwei.,Jiao, Zeren.,...&Song, Kedong.(2021).Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,174,339-351. |
| MLA | Zhu, Jingjing,et al."Evaluation of anti-tumor effects of crocin on a novel 3D tissue-engineered tumor model based on sodium alginate/gelatin microbead".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 174(2021):339-351. |
入库方式： OAI收割
来源：过程工程研究所
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