Engineered Human Heavy-Chain Ferritin with Half-Life Extension and Tumor Targeting by PAS and RGDK Peptide Functionalization
文献类型：期刊论文
| 作者 | Yin, Shuang1; Wang, Yan4; Zhang, Bingyang1; Qu, Yiran1; Liu, Yongdong2; Dai, Sheng3; Zhang, Yao2; Wang, Yingli4; Bi, Jingxiu1 |
| 刊名 | PHARMACEUTICS
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 13期号:4页码:16 |
| 关键词 | ferritin drug delivery tumor targeting half-life extension |
| DOI | 10.3390/pharmaceutics13040521 |
| 英文摘要 | Ferritin, one of the most investigated protein nanocages, is considered as a promising drug carrier because of its advantageous stability and safety. However, its short half-life and undesirable tumor targeting ability has limited its usage in tumor treatment. In this work, two types of functional peptides, half-life extension peptide PAS, and tumor targeting peptide RGDK (Arg-Gly-Asp-Lys), are inserted to human heavy-chain ferritin (HFn) at C-terminal through flexible linkers with two distinct enzyme cleavable sites. Structural characterizations show both HFn and engineered HFns can assemble into nanoparticles but with different apparent hydrodynamic volumes and molecular weights. RGDK peptide enhanced the internalization efficiency of HFn and showed a significant increase of growth inhibition against 4T1 cell line in vitro. Pharmacokinetic study in vivo demonstrates PAS peptides extended ferritin half-life about 4.9 times in Sprague Dawley rats. RGDK peptides greatly enhanced drug accumulation in the tumor site rather than in other organs in biodistribution analysis. Drug loaded PAS-RGDK functionalized HFns curbed tumor growth with significantly greater efficacies in comparison with drug loaded HFn. |
| WOS关键词 | ALBUMIN-BINDING DOMAIN ; CATHEPSIN-B ; NANOPARTICLES ; DELIVERY ; ENCAPSULATION ; SEQUENCE ; THERAPY |
| 资助项目 | University of Adelaide ; Institute of Process Engineering, Chinese Academy of Sciences ; National Natural Science Foundation of China[21576267] ; Beijing Natural Science Foundation[2162041] ; Shanxi Education Science "1331 project" special research project (Research and Development of Traditional Chinese Medicine Micro-emulsion and New Biological Preparation) |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000643517100001 |
| 资助机构 | University of Adelaide ; Institute of Process Engineering, Chinese Academy of Sciences ; National Natural Science Foundation of China ; Beijing Natural Science Foundation ; Shanxi Education Science "1331 project" special research project (Research and Development of Traditional Chinese Medicine Micro-emulsion and New Biological Preparation) |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48574]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Yingli; Bi, Jingxiu |
| 作者单位 | 1.Univ Adelaide, Sch Chem Engn & Adv Mat, Adelaide, SA 5005, Australia 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Brunel Univ London, Dept Chem Engn, Uxbridge UB8 3PH, Middx, England 4.Shanxi Univ Tradit Chinese Med, Sch Chinese Med & Food Engn, Jinzhong 030619, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Shuang,Wang, Yan,Zhang, Bingyang,et al. Engineered Human Heavy-Chain Ferritin with Half-Life Extension and Tumor Targeting by PAS and RGDK Peptide Functionalization[J]. PHARMACEUTICS,2021,13(4):16. |
| APA | Yin, Shuang.,Wang, Yan.,Zhang, Bingyang.,Qu, Yiran.,Liu, Yongdong.,...&Bi, Jingxiu.(2021).Engineered Human Heavy-Chain Ferritin with Half-Life Extension and Tumor Targeting by PAS and RGDK Peptide Functionalization.PHARMACEUTICS,13(4),16. |
| MLA | Yin, Shuang,et al."Engineered Human Heavy-Chain Ferritin with Half-Life Extension and Tumor Targeting by PAS and RGDK Peptide Functionalization".PHARMACEUTICS 13.4(2021):16. |
入库方式： OAI收割
来源：过程工程研究所
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