Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Zhong, Wei1,2; Yang, Maohua1; Hao, Xuemi1,3; Sharshar, Moustafa M.1,3; Wang, Qinhong4; Xing, Jianmin1,3
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| 刊名 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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| 出版日期 | 2021-05-15 |
| 页码 | 11 |
| 关键词 | d‐ lactic acid acetylating acetaldehyde dehydrogenase acetyl‐ CoA metabolic engineering Saccharomyces cerevisiae |
| ISSN号 | 0268-2575 |
| DOI | 10.1002/jctb.6787 |
| 英文摘要 | BACKGROUND d-Lactic acid (d-LA) is gaining increased attention as it can be applied in the food, pharmaceutical, cosmetic, polylactic acid and textile industries. Acid-tolerant Saccharomyces cerevisiae is often engineered to produce organic acids. For the pyruvate decarboxylases (PDCs) or alcohol dehydrogenase 1 (ADH1) disrupted strains, the productivity of d-LA significantly decreases due to the accumulation of acetaldehyde and acetic acid. To overcome the problem, Escherichia coli acetylating acetaldehyde dehydrogenase (A-ALD) enzyme genes (MHPF and EUTE) (EC 1.2.1.10) were integrated into the d-LA producing strain YIP-A15G12 strain (CGMCC2.5803). The function of these genes is to directly convert acetaldehyde into acetyl-CoA without energy consumption. This strain was integrated with Escherichia coli d-lactate dehydrogenase and Issatchenkia orientalis glycosylphosphatidylinositol-anchored protein IoGas1gene and several attenuated key pathway genes, including PDC1, PDC6, ADH1, ADH5, JEN1 (a monocarboxylate transporter), d-lactate dehydrogenase 1 (DLD1), l-lactate cytochrome c oxidoreductase (CYB2) and glycerol-3-phosphate dehydrogenases (GPD1, GPD2). RESULTS The strain expressions of the A-ALD enzymes effectively complemented the attenuated acetaldehyde dehydrogenase (ALD) acetyl-CoA synthetase (ACS) pathway and significantly improved the levels of acetyl-CoA and the productivity of d-LA. Finally, the resulting strainYIP-m-ald6 (CGMCC2.5826) produced 86.9 g L-1 d-LA with a yield of 0.77 g g(-1) glucose and a volumetric productivity of 1.77 g L-1 h(-1) at pH 3.58 under fed-batch fermentation conditions. CONCLUSION Introducing the E. coli acetyl-CoA synthesis pathway into the yeast strain can significantly enhance the growth of the strain and improve the d-LA productivity, as a benefit for d-LA production in industry. (c) 2021 Society of Chemical Industry (SCI). |
| WOS关键词 | EFFICIENT PRODUCTION ; OLEAGINOUS YEAST ; D-LACTATE ; GENE ; GROWTH ; DEHYDROGENASE ; GLUCOSE ; FERMENTATION ; SYNTHETASE ; COENZYME |
| 资助项目 | National Natural Science Foundation of China[21878307] ; National Natural Science Foundation of China[32001039] ; National Natural Science Foundation of China[31800030] ; National Natural Science Foundation of China[31872633] ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission[14ZCZDSY00066] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Chemistry ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000650602400001 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China ; Industrial Biotechnology Program of Tianjin Municipal Science and Technology Commission |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48673]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Qinhong; Xing, Jianmin |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, CAS Key Lab Green Proc & Engn, Beijing, Peoples R China 2.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen Key Lab Synthet Genom,Shenzhen Inst Synt, Guangdong Prov Key Lab Synthet Genom,CAS Key Lab, Shenzhen, Peoples R China 3.Univ Chinese Acad Sci, Coll Chem Engn, Beijing, Peoples R China 4.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, Wei,Yang, Maohua,Hao, Xuemi,et al. Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae[J]. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2021:11. |
| APA | Zhong, Wei,Yang, Maohua,Hao, Xuemi,Sharshar, Moustafa M.,Wang, Qinhong,&Xing, Jianmin.(2021).Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae.JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,11. |
| MLA | Zhong, Wei,et al."Improvement of d-lactic acid productivity by introducing Escherichia coli acetyl-CoA synthesis pathway in engineered Saccharomyces cerevisiae".JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2021):11. |
入库方式： OAI收割
来源：过程工程研究所
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