Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii
文献类型：期刊论文
| 作者 | Zhang, Yuhan1,2; Li, Xiaolian2; Wang, Ziqiang2; Wang, Yunshan2; Ma, Yuanyuan1; Su, Zhiguo2 |
| 刊名 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
 |
| 出版日期 | 2021-05-15 |
| 页码 | 17 |
| 关键词 | Propionibacterium freudenreichii Vitamin B-12 Propionic acid Membrane separation coupled fermentation Metabolic flux analysis |
| ISSN号 | 0273-2289 |
| DOI | 10.1007/s12010-021-03584-y |
| 英文摘要 | The metabolic processes involved in simultaneous production of vitamin B-12 and propionic acid by Propionibacterium freudenreichii are very complicated. To further investigate the regulatory mechanism of this metabolism, a simplified metabolic network was established. The effects of glucose feeding, propionic acid removal, and 5,6-dimethylbenzimidazole (DMB) addition on the metabolic flux distribution were investigated. The results showed that synthesis of propionic acid can be increased by increasing the metabolic flux through the oxaloacetate and methylmalonyl-CoA branches in the early and middle stages of the coupled fermentation. After DMB addition, the synthesis of vitamin B-12 was significantly enhanced via increased metabolic flux through the delta-aminolevulinate branch, which promoted the synthesis of uroporphyrinogen III, a precursor of vitamin B-12. Therefore, the analysis of metabolic flux at key nodes can provide theoretical guidance for the optimization of P. freudenreichii fermentation processes. In an experimental coupled fermentation process, the concentrations of vitamin B-12 and propionic acid reached 21.6 and 50.12 g/L respectively, increased by 105.71% and 73.91% compared with batch fermentation, which provides a new strategy for industrial production. |
| WOS关键词 | MICROBIAL-PRODUCTION ; COBALAMIN VITAMIN-B-12 ; IMMOBILIZED CELLS ; ESCHERICHIA-COLI ; BIOSYNTHESIS ; ACIDIPROPIONICI ; GLYCEROL ; SHERMANII ; BATCH |
| 资助项目 | National Natural Science Foundation of China[21506227] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000650521200001 |
| 出版者 | SPRINGER |
| 资助机构 | National Natural Science Foundation of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48704]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Wang, Ziqiang; Ma, Yuanyuan |
| 作者单位 | 1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,et al. Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2021:17. |
| APA | Zhang, Yuhan,Li, Xiaolian,Wang, Ziqiang,Wang, Yunshan,Ma, Yuanyuan,&Su, Zhiguo.(2021).Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,17. |
| MLA | Zhang, Yuhan,et al."Metabolic Flux Analysis of Simultaneous Production of Vitamin B-12 and Propionic Acid in a Coupled Fermentation Process by Propionibacterium freudenreichii".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021):17. |
入库方式： OAI收割
来源：过程工程研究所
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