Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide
文献类型：期刊论文
| 作者 | Rozhina, Elvira3; Batasheva, Svetlana3; Miftakhova, Regina3; Yan, Xuehai1; Vikulina, Anna4; Volodkin, Dmitry2; Fakhrullin, Rawil3 |
| 刊名 | APPLIED CLAY SCIENCE
 |
| 出版日期 | 2021-05-01 |
| 卷号 | 205页码:10 |
| 关键词 | Halloysite Kaolinite Multiwalled carbon nanotubes Graphene oxide Cytotoxicity Genotoxicity |
| ISSN号 | 0169-1317 |
| DOI | 10.1016/j.clay.2021.106041 |
| 英文摘要 | This study aimed at comparative examining of the interactions between conventionally used clay and carbon nanomaterials and human lung adenocarcinoma cells (A549 cells). The following platy and tubular nanomaterials were tested: carbon nanoparticles, i.e. multi-walled carbon nanotubes (MWCNTs) and graphene oxide nanosheets (GO) as well as nanoclays, i.e. halloysite nanotubes (HNTs) and kaolinite nanosheets (Kaol). Nanoparticle physicochemical properties and their internalization into cells were examined using dynamic light scattering as well as atomic force, 3D laser scanning confocal and darkfield hyperspectral microscopies. Biological aspects of the nanomaterial-cell interaction included assessment of cellular toxicity, DNA damage, metabolic activity, and physical parameters of the cells. Regardless of a shape, carbon nanomaterials demonstrated cell surface adsorption, but negligible penetration into cells compared to nanoclays. However, carbon nanomaterials were found to be the most toxic for cells as probed by the MTS assay. They also turned out to be the most genotoxic for cells compared to nanoclays as revealed by the DNA-Comet assay. GO significantly increased the fraction of apoptotic cells and was the most cytotoxic and genotoxic nanomaterial. Comparison of flow cytometry and MTS data indicated that a cytotoxic effect of MWCNTs was not associated with increased cell death, but was rather due to a decrease in cell metabolic activity and/or proliferation. Finally, no significant effect of the shape of the tested nanomaterials on their internalization and cytotoxicity was revealed. |
| WOS关键词 | DNA-DAMAGE ; IN-VITRO ; OXIDATIVE STRESS ; CELLULAR TOXICITY ; COMET ASSAY ; NANOPARTICLES ; GENOTOXICITY ; DELIVERY ; SURFACE ; ADSORPTION |
| 资助项目 | RFBR[18-53-80067 BRICS_t] ; National Natural Science Fund BRICS STI Framework Program of China[51861145304] |
| WOS研究方向 | Chemistry ; Materials Science ; Mineralogy |
| 语种 | 英语 |
| WOS记录号 | WOS:000647564700006 |
| 出版者 | ELSEVIER |
| 资助机构 | RFBR ; National Natural Science Fund BRICS STI Framework Program of China |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48778]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Fakhrullin, Rawil |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Nottingham Trent Univ, Sch Sci & Technol, Dept Chem & Forens, Clifton Lane, Nottingham NG11 8NS, England 3.Kazan Fed Univ, Inst Fundamental Med & Biol, Kreml Uram 18, Kazan 420008, Republic Of Tat, Russia 4.Fraunhofer Inst Cell Therapy & Immunol, Branch Bioanalyt & Bioproc, Muhlenberg 13, D-14476 Potsdam, Germany |
| 推荐引用方式 GB/T 7714 | Rozhina, Elvira,Batasheva, Svetlana,Miftakhova, Regina,et al. Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide[J]. APPLIED CLAY SCIENCE,2021,205:10. |
| APA | Rozhina, Elvira.,Batasheva, Svetlana.,Miftakhova, Regina.,Yan, Xuehai.,Vikulina, Anna.,...&Fakhrullin, Rawil.(2021).Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide.APPLIED CLAY SCIENCE,205,10. |
| MLA | Rozhina, Elvira,et al."Comparative cytotoxicity of kaolinite, halloysite, multiwalled carbon nanotubes and graphene oxide".APPLIED CLAY SCIENCE 205(2021):10. |
入库方式： OAI收割
来源：过程工程研究所
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