Effect of hydrogen peroxide treatment on the quality of epsilon-poly-L-lysine products
文献类型：期刊论文
| 作者 | Chang, Sen-Lin1,2; Li, Hang1,2; Liu, Jia-Ning1,2; Zhao, Ming-Xia1; Tan, Ming-Hui1,2; Xu, Peng-Wei1,2; Wang, Xiao-dong1; Wang, Li-Wei1; Yuan, Xiao-Fan1; Zhao, Qing-Sheng1
|
| 刊名 | BIOCHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2021-07-01 |
| 卷号 | 171页码:10 |
| 关键词 | epsilon-poly-L-lysine Decoloration Hydrogen peroxide treatment Antibacterial performance |
| ISSN号 | 1369-703X |
| DOI | 10.1016/j.bej.2021.108017 |
| 英文摘要 | epsilon-poly-L-lysine (e-PL) is a novel biopreservative with wide antibacterial spectrum, high safety, and stable property. However, the color of epsilon-PL generating from manufacturing process lowers its appearance and limits its application. This work aimed to decolorize epsilon-PL by hydrogen peroxide. Impacts of hydrogen peroxide concentration, pH, duration time and temperature were discussed. Physicochemical property and bioactivity of epsilon-PL were explored. Results indicated that 43.2 % color saturation and 94.54 % recovery rate were reached when the concentration of hydrogen peroxide, pH, duration time and temperature were 3.0 %, 7.0, 1.5 h and 70 degrees C respectively. Furthermore, the MIC (minimal inhibitory concentration) of the hydrogen peroxide treated epsilon-PL for Bacillus subtilis, Escherichia coli, and Staphylococcus aureus were 2 mg/mL, 0.1 mg/mL, and 0.1 mg/mL respectively, which was similar with untreated epsilon-PL. At last, cytotoxicity test indicated that no cytotoxicity could be observed at concentration lower than 2 mg/mL epsilon-PL. As a result, hydrogen peroxide treatment on epsilon-PL could significantly decolorize epsilon-PL without impairing the antibacterial performance and changing the cytotoxicity. Hydrogen peroxide treatment is a promising method to improve the appearance of products, to reduce the cost of decoloration, and to enlarge the application in food and cosmetics industry. |
| WOS关键词 | PURIFICATION ; SEPARATION ; POLYSACCHARIDES ; PRECIPITATION ; POLYLYSINE |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000648736100014 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/48844]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Zhao, Qing-Sheng; Zhao, Bing |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Sen-Lin,Li, Hang,Liu, Jia-Ning,et al. Effect of hydrogen peroxide treatment on the quality of epsilon-poly-L-lysine products[J]. BIOCHEMICAL ENGINEERING JOURNAL,2021,171:10. |
| APA | Chang, Sen-Lin.,Li, Hang.,Liu, Jia-Ning.,Zhao, Ming-Xia.,Tan, Ming-Hui.,...&Zhao, Bing.(2021).Effect of hydrogen peroxide treatment on the quality of epsilon-poly-L-lysine products.BIOCHEMICAL ENGINEERING JOURNAL,171,10. |
| MLA | Chang, Sen-Lin,et al."Effect of hydrogen peroxide treatment on the quality of epsilon-poly-L-lysine products".BIOCHEMICAL ENGINEERING JOURNAL 171(2021):10. |
入库方式： OAI收割
来源：过程工程研究所
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