Development of purification process for dual-function recombinant human heavy-chain ferritin by the investigation of genetic modification impact on conformation
文献类型：期刊论文
| 作者 | Yin, Shuang1; Zhang, Bingyang1; Lin, Jianying3; Liu, Yongdong2; Su, Zhiguo2; Bi, Jingxiu1 |
| 刊名 | ENGINEERING IN LIFE SCIENCES
 |
| 出版日期 | 2021-06-19 |
| 页码 | 13 |
| 关键词 | ferritin E-helix turnover ferritin genetic modification human heavy-chain ferritin protein characterization protein purification |
| ISSN号 | 1618-0240 |
| DOI | 10.1002/elsc.202000105 |
| 英文摘要 | Ferritin is a promising drug delivery platform and has been functionalized through genetic modifications. This work has designed and expressed a dual-functional engineered human heavy-chain ferritin (HFn) with the inserted functional peptide PAS and RGDK to extend half-life and improve tumor targeted drug delivery. A facile and cost-effective two-step purification pathway for recombinant HFn was developed. The genetic modification was found to affect HFn conformation, and therefore varied the purification performance. Heat-acid precipitation followed by butyl fast flow hydrophobic interaction chromatography (HIC) has been developed to purify HFn and modified HFns. Nucleic acid removal reached above 99.8% for HFn and modified HFns. However, HFn purity reached above 95% and recovery yield (overall) above 90%, compared with modified HFns purity above 82% and recovery yield (overall) above 58%. It is interesting to find that the inserted functional peptides significantly changed the molecule conformation, where a putative turnover of the E-helix with the inserted functional peptides formed a "flop" conformation, in contrast with the "flip" conformation of HFn. It could be the cause of fragile stability of modified HFns, and therefore less tolerant to heat and acid condition, observed by the lower recovery yield in heat-acid precipitation. |
| WOS关键词 | HALF-LIFE ; H-CHAIN ; PROTEINS ; DELIVERY ; BINDING ; NANOPARTICLES ; NANOCARRIERS ; PLATFORM |
| 资助项目 | University of Adelaide ; Institute of Process Engineering, Chinese Academy of Sciences ; National Natural Science Foundation of China[21576267] ; Beijing Natural Science Foundation[2162041] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000663354600001 |
| 出版者 | WILEY |
| 资助机构 | University of Adelaide ; Institute of Process Engineering, Chinese Academy of Sciences ; National Natural Science Foundation of China ; Beijing Natural Science Foundation |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/49198]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Bi, Jingxiu |
| 作者单位 | 1.Univ Adelaide, Sch Chem Engn & Adv Mat, Fac Engn Comp & Math Sci, North Terrace, Adelaide, SA 5005, Australia 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing, Peoples R China 3.Taiyuan Univ Technol, Coll Biomed Engn, Taiyuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Shuang,Zhang, Bingyang,Lin, Jianying,et al. Development of purification process for dual-function recombinant human heavy-chain ferritin by the investigation of genetic modification impact on conformation[J]. ENGINEERING IN LIFE SCIENCES,2021:13. |
| APA | Yin, Shuang,Zhang, Bingyang,Lin, Jianying,Liu, Yongdong,Su, Zhiguo,&Bi, Jingxiu.(2021).Development of purification process for dual-function recombinant human heavy-chain ferritin by the investigation of genetic modification impact on conformation.ENGINEERING IN LIFE SCIENCES,13. |
| MLA | Yin, Shuang,et al."Development of purification process for dual-function recombinant human heavy-chain ferritin by the investigation of genetic modification impact on conformation".ENGINEERING IN LIFE SCIENCES (2021):13. |
入库方式： OAI收割
来源：过程工程研究所
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