Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants
文献类型：期刊论文
| 作者 | Zhang, Hao2; Luo, Jianquan2; Woodley, John M.1; Wan, Yinhua2 |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2021-10-01 |
| 卷号 | 421页码:12 |
| ISSN号 | 1385-8947 |
| 关键词 | Micropollutants Biocatalytic membrane Enzyme mobility Enzyme immobilization Confinement strength |
| DOI | 10.1016/j.cej.2020.127870 |
| 英文摘要 | Enzymes in living cells are highly dynamic but at the same time regularly confined for achieving efficient metabolism. Inspired by this phenomenon, we have prepared a novel biocatalytic membrane with high enzyme activity and stability by tuning the confinement strength of the membrane to enzyme, which was achieved via modifying the support layer of a polymeric nanofiltration (NF) membrane and reversely filtrating enzyme. A mussel-inspired coating was used to modify the support interior of the NF membrane to enhance charge and steric effects on enzyme, thus stabilizing enzyme in the membrane with little increment in mass transfer resistance for substrate and products (only 20% permeability loss with a high enzyme loading of 1.34 mg/cm2). A suitable confinement strength of the membrane to enzyme could delay the enzyme leakage and endow enzyme with certain mobility for efficient reaction. Thus, the obtained biocatalytic membrane exhibited a negligible decline in BPA removal efficiency for 7 reuse cycles (<3.5%) or 36 h continuous operation (<1%) in flow through mode, resulting in a long-term stability adequate for micropollutant removal. For the first time, enzyme mobility was defined and calculated to quantify the confinement strength of the membrane, which could be used to optimize the microenvironment for enzyme immobilization and predict the performance of the biocatalytic membrane. This work concluded that rationally regulating the enzyme mobility in the membrane and a periodic back-flushing operation for redistribution of enzymes could achieve a long-term stable removal of micropollutant in water by a biocatalytic membrane. |
| WOS关键词 | ORGANIC MICROPOLLUTANTS ; BISPHENOL-A ; IMMOBILIZATION ; LACCASE ; PERFORMANCE ; STABILITY |
| 资助项目 | National Natural Science Foundation of China[21878306] ; Beijing Natural Science Foundation[2192053] |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000663713700005 |
| 资助机构 | National Natural Science Foundation of China ; Beijing Natural Science Foundation |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/49235]  |
| 专题 | 中国科学院过程工程研究所 |
| 通讯作者 | Luo, Jianquan |
| 作者单位 | 1.Tech Univ Denmark, Dept Chem & Biochem Engn, DK-2800 Lyngby, Denmark 2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hao,Luo, Jianquan,Woodley, John M.,et al. Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants[J]. CHEMICAL ENGINEERING JOURNAL,2021,421:12. |
| APA | Zhang, Hao,Luo, Jianquan,Woodley, John M.,&Wan, Yinhua.(2021).Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants.CHEMICAL ENGINEERING JOURNAL,421,12. |
| MLA | Zhang, Hao,et al."Confining the motion of enzymes in nanofiltration membrane for efficient and stable removal of micropollutants".CHEMICAL ENGINEERING JOURNAL 421(2021):12. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。