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Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode

文献类型：期刊论文

作者Liang, Xinquan1,2; Li, Chenglin1,2; Cao, Weifeng2,3; Cao, Weilei2,3; Shen, Fei2,3; Wan, Yinhua2,3
刊名MOLECULES
出版日期2021-07-01
卷号26期号:13页码:17
关键词fructo-oligosaccharides dissolved oxygen concentration repeated-batch culture Aureobasidium pullulans
DOI10.3390/molecules26133867
英文摘要Fructo-oligosaccharides (FOS) are prebiotics with numerous health benefits. So far, the dissolved oxygen (DO) concentration control strategy for fermentative production of FOS is still unknown. In order to improve FOS production, the effects of DO concentration and fermentation mode on FOS using Aureobasidium pullulans were investigated in this study. The greatest FOS production (123.2 +/- 6.2 g/L), with a yield of 61.6% +/- 3.0% (g FOS/g sucrose), was obtained in batch culture under high DO concentration. Furthermore, repeated-batch culture revealed that enzyme production and FOS production were not closely associated with cell growth. By keeping the DO concentration above 5% in the repeated-batch culture, a maximum FOS concentration of 548.3 +/- 37.4 g/L and yield of 68.6% +/- 2.6% (g FOS/g sucrose) were obtained, which were 3.45% and 11.4% times higher than those obtained in the batch culture without DO control, respectively. Additionally, the ratios of 1-fructofuranosyl nystose (GF4) and 1,1,1,1-kestohexose (GF5) were 33.8% and 23.2%, respectively, in the product of repeated-batch culture, but these compounds were not detected in batch culture. Thus, it can be concluded that the DO concentration affects not only the yield of FOS but also the composition of FOS with different degrees of polymerization, which is the key factor in the fermentative production of FOS with a high polymerization degree.
WOS关键词BETA-FRUCTOFURANOSIDASE ; FRUCTOSYLTRANSFERASE ; ENZYME ; STRATEGY ; CULTURE
资助项目Beijing Natural Science Foundation, China[5182025] ; Science and Technology Service Network Program of Chinese Academy of Sciences[KFJ-STS-QYZX-096] ; National Natural Science Foundation of China, China[21406240] ; National High Technology Research and Development Program of China[2015AA021002] ; National High Technology Research and Development Program of China[2014AA021005]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000670974700001
出版者MDPI
资助机构Beijing Natural Science Foundation, China ; Science and Technology Service Network Program of Chinese Academy of Sciences ; National Natural Science Foundation of China, China ; National High Technology Research and Development Program of China
源URL[http://ir.ipe.ac.cn/handle/122111/49351]  
专题中国科学院过程工程研究所
通讯作者Cao, Weifeng
作者单位1.Guangxi Univ, Dept Sugar Engn, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China
3.Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Liang, Xinquan,Li, Chenglin,Cao, Weifeng,et al. Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode[J]. MOLECULES,2021,26(13):17.
APA Liang, Xinquan,Li, Chenglin,Cao, Weifeng,Cao, Weilei,Shen, Fei,&Wan, Yinhua.(2021).Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode.MOLECULES,26(13),17.
MLA Liang, Xinquan,et al."Fermentative Production of Fructo-Oligosaccharides Using Aureobasidium pullulans: Effect of Dissolved Oxygen Concentration and Fermentation Mode".MOLECULES 26.13(2021):17.

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来源：过程工程研究所

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