Comparative Metagenomics of the Active Layer and Permafrost from Low-Carbon Soil in the Canadian High Arctic
文献类型：期刊论文
| 作者 | Wu, Xiaofen1; Chauhan, Archana1,2; Layton, Alice C.1; Vetter, Maggie C. Y. Lau3,4 ; Stackhouse, Brandon T.3; Williams, Daniel E.1; Whyte, Lyle5; Pfiffner, Susan M.1; Onstott, Tullis C.3; Vishnivetskaya, Tatiana A.1
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2021-09-21 |
| 卷号 | 55期号:18页码:12683-12693 |
| 关键词 | Arctic permafrost active layer 16S rRNA metagenome-assembled genome metabolism |
| ISSN号 | 0013-936X |
| DOI | 10.1021/acs.est.1c00802 |
| 英文摘要 | Approximately 87% of the Arctic consists of low-organic carbon mineral soil, but knowledge of microbial activity in low-carbon permafrost (PF) and active layer soils remains limited. This study investigated the taxonomic composition and genetic potential of microbial communities at contrasting depths of the active layer (5, 35, and 65 cm below surface, bls) and PF (80 cm bls). We showed microbial communities in PF to be taxonomically and functionally different from those in the active layer. 16S rRNA gene sequence analysis revealed higher biodiversity in the active layer than in PF, and biodiversity decreased significantly with depth. The reconstructed 91 metagenome-assembled genomes showed that PF was dominated by heterotrophic, fermenting Bacteroidota using nitrite as their main electron acceptor. Prevalent microbes identified in the active layer belonged to bacterial taxa, gaining energy via aerobic respiration. Gene abundance in metagenomes revealed enrichment of genes encoding the plantderived polysaccharide degradation and metabolism of nitrate and sulfate in PF, whereas genes encoding methane/ammonia oxidation, cold-shock protein, and two-component systems were generally more abundant in the active layer, particularly at 5 cm bls. The results of this study deepen our understanding of the low-carbon Arctic soil microbiome and improve prediction of the impacts of thawing PF. |
| WOS关键词 | COMMUNITY STRUCTURE ; CYCLE FEEDBACKS ; CLIMATE ; OMICS ; THAW |
| 资助项目 | U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science Program[DE-SC0004902] ; U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science Program[DE-SC0020369] ; National Science Foundation[DEB-1442262] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000699957900052 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science Program ; National Science Foundation |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/8862]  |
| 专题 | 深海科学研究部_地外海洋系统研究室 |
| 通讯作者 | Vishnivetskaya, Tatiana A. |
| 作者单位 | 1.Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37996 USA 2.Panjab Univ, Dept Zool, Chandigarh 160014, India 3.Princeton Univ, Dept Geosci, Princeton, NJ 08544 USA 4.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Lab Extraterr Ocean Syst, Sanya, Hainan, Peoples R China 5.McGill Univ, Dept Nat Resource Sci, Ste Anne De Bellevue, PQ H9X 3V9, Canada |
| 推荐引用方式 GB/T 7714 | Wu, Xiaofen,Chauhan, Archana,Layton, Alice C.,et al. Comparative Metagenomics of the Active Layer and Permafrost from Low-Carbon Soil in the Canadian High Arctic[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2021,55(18):12683-12693. |
| APA | Wu, Xiaofen.,Chauhan, Archana.,Layton, Alice C..,Vetter, Maggie C. Y. Lau.,Stackhouse, Brandon T..,...&Vishnivetskaya, Tatiana A..(2021).Comparative Metagenomics of the Active Layer and Permafrost from Low-Carbon Soil in the Canadian High Arctic.ENVIRONMENTAL SCIENCE & TECHNOLOGY,55(18),12683-12693. |
| MLA | Wu, Xiaofen,et al."Comparative Metagenomics of the Active Layer and Permafrost from Low-Carbon Soil in the Canadian High Arctic".ENVIRONMENTAL SCIENCE & TECHNOLOGY 55.18(2021):12683-12693. |
入库方式： OAI收割
来源：深海科学与工程研究所
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