Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine
文献类型：期刊论文
| 作者 | Zhu, Fang-Chao1,2 ; Lian, Chun-Ang1,2; He, Li-Sheng1
|
| 刊名 | MICROORGANISMS
 |
| 出版日期 | 2020-12-01 |
| 卷号 | 8期号:12页码:16 |
| 关键词 | deep-sea holothurian gut symbiont CRISPR Tenericutes |
| DOI | 10.3390/microorganisms8121874 |
| 英文摘要 | Intestinal bacterial communities are highly relevant to the digestion, nutrition, growth, reproduction, and immunity of animals, but little is known about the composition and function of intestinal microbiota in deep-sea invertebrates. In this study, the intestinal microbiota of six holothurian Molpadia musculus were investigated, showing that their midguts were predominantly occupied by Izemoplasmatales bacteria. Using metagenomic sequencing, a draft genome of 1,822,181 bp was successfully recovered. After comparison with phylogenetically related bacteria, genes involved in saccharide usage and de novo nucleotide biosynthesis were reduced. However, a set of genes responsible for extracellular nucleoside utilization and 14 of 20 amino acid synthesis pathways were completely retained. Under oligotrophic condition, the gut-associated bacterium may make use of extracellular DNA for carbon and energy supplement, and may provide essential amino acids to the host. The clustered regularly interspaced short palindromic repeat (CRISPR) and restriction-modification (RM) systems presented in the genome may provide protection against invading viruses. A linear azol(in)e-containing peptide gene cluster for bacteriocin synthesize was also identified, which may inhibit the colonization and growth of harmful bacteria. Known virulence factors were not found by database searching. On the basis of its phylogenetic position and metabolic characteristics, we proposed that the bacterium represented a novel genus and a novel family within the Izemoplasmatales order and suggested it be named "Candidatus Bathyoplasma sp. NZ". This was the first time describing host-associated Izemoplasmatales. |
| WOS关键词 | MULTIPLE SEQUENCE ALIGNMENT ; ORGANIC-MATTER ; PHYLOGENOMIC ANALYSIS ; GUT ; CONTRACTILE ; ANNOTATION ; PREDICTION ; ALGORITHM ; EVOLUTION ; QUALITY |
| 资助项目 | National Key Research and Development Program of China[2018YFC0309804] ; National Key Research and Development Program of China[2016YFC0302504] ; National Key Research and Development Program of China[2016YFC0304905] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000602533700001 |
| 出版者 | MDPI |
| 资助机构 | National Key Research and Development Program of China |
| 源URL | [http://ir.idsse.ac.cn/handle/183446/8475]  |
| 专题 | 研究生部 深海科学研究部_深海生物学研究室_深海生物蛋白质组学及分子生物学研究组 |
| 通讯作者 | He, Li-Sheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 101408, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Fang-Chao,Lian, Chun-Ang,He, Li-Sheng. Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine[J]. MICROORGANISMS,2020,8(12):16. |
| APA | Zhu, Fang-Chao,Lian, Chun-Ang,&He, Li-Sheng.(2020).Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine.MICROORGANISMS,8(12),16. |
| MLA | Zhu, Fang-Chao,et al."Genomic Characterization of a Novel Tenericutes Bacterium from Deep-Sea Holothurian Intestine".MICROORGANISMS 8.12(2020):16. |
入库方式： OAI收割
来源：深海科学与工程研究所
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