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Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2

文献类型：期刊论文

作者Yang, Qiang1,2; Zheng, Zhiming2; Zhao, Genhai2; Wang, Li1,2; Wang, Han2; Ni, Wenfeng1,2; Sun, Xiaowen1,2; Zhang, Mengxue1,2; Tang, Hengfang1,2; Wang, Peng2
刊名PROTEIN EXPRESSION AND PURIFICATION
出版日期2022
卷号189
ISSN号1046-5928
关键词Elizabethkingia meningoseptica Geranylgeranyl diphosphate synthase Soluble expression MK-4
DOI10.1016/j.pep.2021.105986
通讯作者Zheng, Zhiming(zhengzhiming2014@163.com) ; Wang, Peng(pengwang@ipp.ac.cn)
英文摘要To date, there is no functional characterization of EmGGPPS (from Elizabethkingia meningoseptica sp.F2) as enzymes catalyzing GGPP. In this research, maltose-binding protein (MBP), disulfide bond A (DbsA), disulfide bond C (DbsC), and two other small protein tags, GB1 (Protein G B1 domain) and ZZ (Protein A IgG ZZ repeat domain), were used as fusion partners to construct an EmGGPPS fusion expression system. The results indicated that the expression of MBP-EmGGPPS was higher than that of the other four fusion proteins in E. coli BL21 (DE3). Additionally, using EmGGPPS as a catalyst for the production of GGPP was verified using a color complementation assay in Escherichia coli. In parallel with it, the enzyme activity experiment in vitro showed that the EmGGPPS protein could produce GGPP, GPP and FPP. Finally, we successfully demonstrated MK-4 production in engineered E. coli by overexpression of EmGGPPS.
WOS关键词ESCHERICHIA-COLI ; ISOPRENOID BIOSYNTHESIS ; PROTEIN EXPRESSION ; BINDING DOMAIN ; VITAMIN-K ; PURIFICATION ; SYNTHASE ; PATHWAY ; YEAST
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号WOS:000709874400002
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/125762]  
专题中国科学院合肥物质科学研究院
通讯作者Zheng, Zhiming; Wang, Peng
作者单位1.Univ Sci & Technol China, Hefei 230026, Peoples R China
2.Chinese Acad Sci, Inst Intelligent Machines, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Qiang,Zheng, Zhiming,Zhao, Genhai,et al. Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2[J]. PROTEIN EXPRESSION AND PURIFICATION,2022,189.
APA Yang, Qiang.,Zheng, Zhiming.,Zhao, Genhai.,Wang, Li.,Wang, Han.,...&Wang, Peng.(2022).Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2.PROTEIN EXPRESSION AND PURIFICATION,189.
MLA Yang, Qiang,et al."Cloning and functional characterization of the geranylgeranyl diphosphate synthase(GGPPS)from Elizabethkingia meningoseptica sp.F2".PROTEIN EXPRESSION AND PURIFICATION 189(2022).

入库方式： OAI收割

来源：合肥物质科学研究院

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