One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip
文献类型：期刊论文
| 作者 | Gao, Nailong1,2; Chang, Jianguo2; Dai, Peng3; Zhu, Ziming3; You, Hui3
|
| 刊名 | ANALYTICAL SCIENCES
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 37 |
| ISSN号 | 0910-6340 |
| 关键词 | Power-free one-step cancer biomarker POCT microfluidic immunoassay microchip |
| DOI | 10.2116/analsci.21P098 |
| 通讯作者 | You, Hui(usmlhy@iim.ac.cn) |
| 英文摘要 | Alpha-fetoprotein (AFP) is an important disease biomarker, relating to cancers such as hepatocarcinomas and gastric cancer. However, traditional methods are time-consuming, relied on bulky instruments and trained professionals, cannot satisfy the demand for low cost and point-of-care testing (POCT). In this study, a power-free POCT device was developed for the rapid and low-cost detection of AFP via one-sampling. Based on the principle of sandwich immunofluorescence, the chip is capable of automatically accomplishing on-chip mixing, labeling and capturing procedures, which only require that operator add 40 mu L sample into the chip one time. The proposed device is capable of sensitively detecting human AFP in FBS with a dynamic range of 10 - 1000 ng/mL and LOD (1.88 ng/mL) within a short time of 3 min. Predictably, our method holds a great potential to be applied in the POC diagnostics of proteins, especially for some regions that are resource-limited. |
| WOS关键词 | OF-CARE DETECTION ; IMMUNOASSAY ; DEVICES |
| 资助项目 | Key Research and Development Plan Project of Anhui Province[1704a0802157] ; Key-Area Research and Development Program of Guangdong Province[2019B020219003] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | JAPAN SOC ANALYTICAL CHEMISTRY |
| WOS记录号 | WOS:000730243700007 |
| 资助机构 | Key Research and Development Plan Project of Anhui Province ; Key-Area Research and Development Program of Guangdong Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/126438]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | You, Hui |
| 作者单位 | 1.Univ Sci & Technol China, Dept Precis Machinery & Precis Instrumentat, Hefei 230026, Peoples R China 2.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China 3.Guangxi Univ, Sch Mech Engn, Nanning 530004, Guangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Nailong,Chang, Jianguo,Dai, Peng,et al. One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip[J]. ANALYTICAL SCIENCES,2021,37. |
| APA | Gao, Nailong,Chang, Jianguo,Dai, Peng,Zhu, Ziming,&You, Hui.(2021).One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip.ANALYTICAL SCIENCES,37. |
| MLA | Gao, Nailong,et al."One-sampling and Rapid Analysis of Cancer Biomarker on a Power-free and Low-cost Microfluidic Chip".ANALYTICAL SCIENCES 37(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。