Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T
文献类型：期刊论文
| 作者 | Fu, Wenqiang2,3; Jing, Haitao2,3; Xu, Xiaojuan2,3; Xu, Suping2; Wang, Tao2 ; Hu, Wenxuan2,3; Li, Huihui2,3; Zhang, Na1,2,4,5
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| 刊名 | NUCLEIC ACIDS RESEARCH
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| 出版日期 | 2021-10-11 |
| 卷号 | 49 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gkab755 |
| 通讯作者 | Zhang, Na(nazhang@hmfl.cas.cn) |
| 英文摘要 | The final 3'-terminal residue of the telomeric DNA G-overhang is inherently less precise. Here, we describe how alteration of the last 3'-terminal base affects the mutual recognition between two different G-rich oligomers of human telomeric DNA in the formation of heteromolecular G-quadruplexes (heteroGC/s). Associations between three- and single-repeat fragments of human telomeric DNA, target d(GGGTTAGGGTTAGGG) and probe d(TAGGGT), in Na+ solution yield two coexisting forms of (3 + 1) hybrid hetero-GQs: the kinetically favourable LLP-form (left loop progression) and the thermodynamically controlled RLP-form (right loop progression). However, only the adoption of a single LLP-form has been previously reported between the same probe d(TAGGGT) and a target variant d(GGGTTAGGGTTAGGG1) having one extra 3'-end thymine. Moreover, the flanking base alterations of short G-rich probe variants also significantly affect the loop progressions of hetero-GQs. Although seemingly two pseudo-mirror counter partners, the RLP-form exhibits a preference over the LLP-form to be recognized by a low equivalent of fluorescence dye thioflavin T (ThT). To a greater extent, ThT preferentially binds to RLP hetero-GO than with the corresponding telomeric DNA duplex context or several other representative unimolecular GQs. |
| WOS关键词 | MOLECULAR-STRUCTURE DETERMINATION ; K+ SOLUTION ; XPLOR-NIH ; SELECTIVE RECOGNITION ; HEXANUCLEOTIDE REPEAT ; DNA ; NMR ; SEQUENCE ; KINETICS ; BINDING |
| 资助项目 | National Key Research and Development Program of China[2016YFA0400900] ; National Natural Science Foundation of China[U1932157] ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology[2018ZYFX004] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000715870700040 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Key Research and Development Program of China ; National Natural Science Foundation of China ; Major/Innovative Program of Development Foundation of Hefei Center for Physical Science and Technology |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/126564]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Na |
| 作者单位 | 1.Key Lab Anhui Prov High Field Magnet Resonance Im, Hefei 230031, Peoples R China 2.Chinese Acad Sci, High Magnet Field Lab, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Peoples R China 5.High Magnet Field Lab Anhui Prov, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Wenqiang,Jing, Haitao,Xu, Xiaojuan,et al. Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T[J]. NUCLEIC ACIDS RESEARCH,2021,49. |
| APA | Fu, Wenqiang.,Jing, Haitao.,Xu, Xiaojuan.,Xu, Suping.,Wang, Tao.,...&Zhang, Na.(2021).Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T.NUCLEIC ACIDS RESEARCH,49. |
| MLA | Fu, Wenqiang,et al."Two coexisting pseudo-mirror heteromolecular telomeric G-quadruplexes in opposite loop progressions differentially recognized by a low equivalent of Thioflavin T".NUCLEIC ACIDS RESEARCH 49(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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