A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs
文献类型：期刊论文
| 作者 | Zhao, Jun2; Wu, Jiandong4; Wu, Xiaosong2,3 ; Deng, Guoqing2; Liu, Yong2; Lin, Francis1; Zhu, Ling2
|
| 刊名 | CHEMICAL COMMUNICATIONS
 |
| 出版日期 | 2021-11-25 |
| 卷号 | 57 |
| ISSN号 | 1359-7345 |
| DOI | 10.1039/d1cc05522d |
| 通讯作者 | Lin, Francis(Francis.Lin@umanitoba.ca) ; Zhu, Ling(zhul@aiofm.ac.cn) |
| 英文摘要 | We designed an efficient self-quenching fluorescence probe and constructed this probe-mediated exponential isothermal amplification system for miRNA detection. Owing to the significant improvement in the detective signal-to-background ratio, a wide dynamic range of 9 orders of magnitude and a limit of detection as low as 0.08 aM can be easily achieved in a single step. Furthermore, benefiting from the additional advantages of high specificity and biocompatibility, the proposed method has been demonstrated to be capable of accurately quantifying miRNA biomarkers in serum, which will provide promising perspectives for clinical diagnosis. |
| WOS关键词 | ROLLING CIRCLE AMPLIFICATION ; UNIVERSAL REPORTER ; SERUM |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | ROYAL SOC CHEMISTRY |
| WOS记录号 | WOS:000719433900001 |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/126600]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Lin, Francis; Zhu, Ling |
| 作者单位 | 1.Univ Manitoba, Winnipeg, MB R3T 2N2, Canada 2.Chinese Acad Sci, Ctr Engn Technol Res Biomed Opt Instrument, Hefei Inst Phys Sci, Hefei 230031, Peoples R China 3.Univ Sci & Technol China, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Shenzhen Inst Adv Technol, Inst Biomed & Hlth Engn, Shenzhen 518055, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jun,Wu, Jiandong,Wu, Xiaosong,et al. A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs[J]. CHEMICAL COMMUNICATIONS,2021,57. |
| APA | Zhao, Jun.,Wu, Jiandong.,Wu, Xiaosong.,Deng, Guoqing.,Liu, Yong.,...&Zhu, Ling.(2021).A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs.CHEMICAL COMMUNICATIONS,57. |
| MLA | Zhao, Jun,et al."A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and specific detection of microRNAs".CHEMICAL COMMUNICATIONS 57(2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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