Role of FUS-CHOP in Myxoid Liposarcoma via miR-486/CDK4 Axis
文献类型：期刊论文
| 作者 | Wu, Hao ; Xia, Liming; Xu, Haichao
|
| 刊名 | BIOCHEMICAL GENETICS
 |
| 出版日期 | 2021-11-18 |
| 关键词 | FUS-CHOP Myxoid liposarcoma Apoptosis miR-486 CDK4 axis |
| ISSN号 | 0006-2928 |
| DOI | 10.1007/s10528-021-10151-x |
| 通讯作者 | Xia, Liming(drlmx1340@163.com) |
| 英文摘要 | This study aimed to explore the roles and relationship between FUsed in Sarcoma (FUS)-C/EBP HOmologous Protein (CHOP), microRNA (miR)-486 and cyclin dependent kinase 4 (CDK4) in myxoid liposarcoma, and determined whether FUS-CHOP can regulate proliferation and apoptosis of myxoid liposarcoma cells by regulating miR-486/CDK4 axis. The levels of miR-486, CDK4 and FUS-CHOP in myxoid liposarcoma samples/adjacent normal muscle tissues and myxoid liposarcoma/human adipose-derived stem cell line were evaluated using reverse transcription-quantitative polymerase chain reaction and western blotting. Cell proliferation and apoptosis were performed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide and flow cytometry, respectively. Furthermore, the apoptosis-related proteins were determined using Western blot assay. We found that miR-486 was down-regulated, FUS-CHOP and CDK4 were up-regulated in myxoid liposarcoma tissues and myxoid liposarcoma cell lines. Moreover, FUS-CHOP-siRNA distinctly suppressed FUS-CHOP level and increased miR-486 levels in 1955/91 cells. Our results demonstrated that knockdown of FUS-CHOP by siRNA inhibited 1955/91 growth, promoted cell apoptosis and enhanced cleaved Caspase3 protein expression. However, all these data were reversed by miR-486 inhibitor. Similarly, compared to mimic control, miR-486 mimic markedly reduced 1955/91 cells growth, induced cell apoptosis and fortified cleaved Caspase3 level, while these results were abolished by CDK4-plasmid. Collectively, our observations clearly suggested that FUS-CHOP regulated myxoid liposarcoma cell proliferation and apoptosis by the regulation of miR-486/CDK4 axis, indicating the potential use of FUS-CHOP-siRNA as a promising therapy for myxoid liposarcoma. |
| WOS关键词 | EXPRESSION ; MALIGNANCY ; REGULATOR ; CANCER ; CDK4 |
| 资助项目 | Zhejiang Medical and Health Science and Technology Plan Project[2017KY249] ; Zhejiang Medical and Health Science and Technology Plan Project[2019KY332] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000720201200003 |
| 出版者 | SPRINGER/PLENUM PUBLISHERS |
| 资助机构 | Zhejiang Medical and Health Science and Technology Plan Project |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/126608]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Xia, Liming |
| 作者单位 | Chinese Acad Sci, Inst Basic Med & Canc IBMC, Bone & Soft Tissue Sarcoma Dept, Canc Hosp,Univ Chinese Acad Sci,Zhejiang Canc Hos, 1 Banshan East Rd, Hangzhou 310022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Hao,Xia, Liming,Xu, Haichao. Role of FUS-CHOP in Myxoid Liposarcoma via miR-486/CDK4 Axis[J]. BIOCHEMICAL GENETICS,2021. |
| APA | Wu, Hao,Xia, Liming,&Xu, Haichao.(2021).Role of FUS-CHOP in Myxoid Liposarcoma via miR-486/CDK4 Axis.BIOCHEMICAL GENETICS. |
| MLA | Wu, Hao,et al."Role of FUS-CHOP in Myxoid Liposarcoma via miR-486/CDK4 Axis".BIOCHEMICAL GENETICS (2021). |
入库方式： OAI收割
来源：合肥物质科学研究院
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