Downregulation of hsa-miR-203 in peripheral blood and wound margin tissue by negative pressure wound therapy contributes to wound healing of diabetic foot ulcers
文献类型：期刊论文
| 作者 | Liu, Lei2 ; Chen, Ruofei3; Jia, Zeguo2; Li, Xueting2; Tang, Ying2; Zhao, Xiaotong2; Zhang, Shiqi2; Luo, Li2; Fang, Zhaohui1; Zhang, Yuanzhi4
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| 刊名 | MICROVASCULAR RESEARCH
 |
| 出版日期 | 2022 |
| 卷号 | 139 |
| 关键词 | Type 2 diabetes Foot ulcers Negative pressure wound therapy Keratinocytes Hsa-miR-203 p63 |
| ISSN号 | 0026-2862 |
| DOI | 10.1016/j.mvr.2021.104275 |
| 通讯作者 | Chen, Mingwei(chmw1@163.com) |
| 英文摘要 | Negative pressure wound therapy (NPWT) has been widely used in the treatment of chronic wounds, including diabetic foot ulcers (DFU) as the severe manifestation of diabetic foot. Hsa-miR-203 is proven to be correlated with the severity of DFU. To investigate whether NPWT influences hsa-miR-203 levels in persons with DFU, we detected hsa-miR-203 levels in peripheral plasma and wound margin tissue from the following patients: type 2 diabetic (T2D) patients with DFU (DFU group), T2D patients without DFU (NDFU group), patients with chronic skin ulcer and normal glucose tolerance (SUC group), and healthy volunteers with normal glucose tolerance (NC group). All patients in SUC group received NPWT. As contrast, some of patients in DFU group received NPWT (NPWT group) while others chose routine dressing therapy (non-NPWT group). In vitro experiments were also performed to determine influences of negative pressure on cell proliferation and migration of HaCaT cells (human keratinocytes). Results showed that before NPWT, levels of hsa-miR-203 in peripheral plasma (P-miR203) and wound margin tissue (T-miR-203) of DFU group were obviously increased compared to SUC group while expression of P-miR-203 decreased in NDFU group compared with NC group. After NPWT, levels of P-miR203 and T-miR-203 in DFU and SUC group were significantly lower than before. Changes of P-miR-203 and TmiR-203 after NPWT were positively correlated with 4-week ulcer healing rate in NPWT and SUC group. In vitro, negative pressure lowered the expression of hsa-miR-203, enhancing cell proliferation and migration in HaCaT cells via up-regulation of p63 protein. Meanwhile, the effects of negative pressure on cells were remarkable reduced by high-glucose intervention. Our study suggests that NPWT promotes DFU healing by reducing the expression of hsa-miR-203 in peripheral blood and wound tissue. The changes of hsa-miR-203 in peripheral blood and wound tissue may be related to the therapeutic effect of NPWT. |
| WOS关键词 | VACUUM-ASSISTED CLOSURE ; EXPRESSION ; SKIN ; MICRORNA-203 ; DIFFERENTIATION ; MANAGEMENT ; UPDATE ; SERUM |
| 资助项目 | Key Research and Development Program of Anhui Province[202004a07020016] ; Natural Science Foundation of Anhui Province[2108085MH269] |
| WOS研究方向 | Cardiovascular System & Cardiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000717852800004 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | Key Research and Development Program of Anhui Province ; Natural Science Foundation of Anhui Province |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/126690]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Chen, Mingwei |
| 作者单位 | 1.Anhui Univ Chinese Med, Affiliated Hosp 1, Dept Endocrinol, Hefei 230031, Peoples R China 2.Anhui Med Univ, Affiliated Hosp 1, Dept Endocrinol, 218 Jixi Rd,Shushan Dist, Hefei 230022, Peoples R China 3.Anhui Med Univ, Affiliated Hosp 1, Dept Rheumatol & Immunol, Hefei 230022, Peoples R China 4.Chinese Acad Sci, Hefei Inst Phys Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Lei,Chen, Ruofei,Jia, Zeguo,et al. Downregulation of hsa-miR-203 in peripheral blood and wound margin tissue by negative pressure wound therapy contributes to wound healing of diabetic foot ulcers[J]. MICROVASCULAR RESEARCH,2022,139. |
| APA | Liu, Lei.,Chen, Ruofei.,Jia, Zeguo.,Li, Xueting.,Tang, Ying.,...&Chen, Mingwei.(2022).Downregulation of hsa-miR-203 in peripheral blood and wound margin tissue by negative pressure wound therapy contributes to wound healing of diabetic foot ulcers.MICROVASCULAR RESEARCH,139. |
| MLA | Liu, Lei,et al."Downregulation of hsa-miR-203 in peripheral blood and wound margin tissue by negative pressure wound therapy contributes to wound healing of diabetic foot ulcers".MICROVASCULAR RESEARCH 139(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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