The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
文献类型：期刊论文
| 作者 | Andrzejczak, Olga A.; Havelund, Jesper F.; Wang, Wei-Qing; Kovalchuk, Sergey6; Hagensen, Christina E.; Hasler-Sheetal, Harald; Jensen, Ole N.; Rogowska-Wrzesinska, Adelina; Moller, Ian Max4; Hebelstrup, Kim H. |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2020 |
| 卷号 | 21期号:4 |
| 关键词 | ethylene anaerobiosis hemoglobin histones N-end rule stress priming |
| DOI | 10.3390/ijms21041546 |
| 文献子类 | Article |
| 英文摘要 | Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activates transcription factors that are regulated by levels of NO and O-2 in the N-end rule pathway. To map the cellular and metabolic effects of hypoxia in barley (Hordeum vulgare L., cv. Golden Promise), with or without priming to hypoxia, we studied the proteome and metabolome of wild type (WT) and hemoglobin overexpressing (HO) plants in normoxia and after 24 h hypoxia (WT24, HO24). The WT plants were more susceptible to hypoxia than HO plants. The chlorophyll a + b content was lowered by 50% and biomass by 30% in WT24 compared to WT, while HO plants were unaffected. We observed an increase in ROS production during hypoxia treatment in WT seedlings that was not observed in HO seedlings. We identified and quantified 9694 proteins out of which 1107 changed significantly in abundance. Many proteins, such as ion transporters, Ca2+-signal transduction, and proteins related to protein degradation were downregulated in HO plants during hypoxia, but not in WT plants. Changes in the levels of histones indicates that chromatin restructuring plays a role in the priming of hypoxia. We also identified and quantified 1470 metabolites, of which the abundance of >500 changed significantly. In summary the data confirm known mechanisms of hypoxia priming by ethylene priming and N-end rule activation; however, the data also indicate the existence of other mechanisms for hypoxia priming in plants. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1422-0067 |
| 出版地 | BASEL |
| WOS关键词 | NITRIC-OXIDE ; OXIDATIVE STRESS ; LOW-OXYGEN ; MASS-SPECTROMETRY ; ARABIDOPSIS ; PLANTS ; ROOTS ; NO ; BIOSYNTHESIS ; RESPONSES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000522524400373 |
| 资助机构 | Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0052451] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19084]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.[Andrzejczak, Olga A.; Hebelstrup, Kim H.] Aarhus Univ, Dept Agroecol, Sect Crop Genet & Biotechnol, Forsogsvej 1, DK-4200 Slagelse, Denmark 2.Univ Southern Denmark, Dept Biochem & Mol Biol, Campusvej 55, DK-5230 Odense M, Denmark 3.Univ Southern Denmark, VILLUM Ctr Bioanalyt Sci, Campusvej 55, DK-5230 Odense M, Denmark 4.Univ Southern Denmark, Dept Biol, Nordcee, Campusvej 55, DK-5230 Odense M, Denmark 5.Aarhus Univ, Dept Mol Biol & Genet, Forsogsvej 1, DK-4200 Slagelse, Denmark 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.RAS, Lab Bioinformat Methods Combinatorial Chem & Biol, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia |
| 推荐引用方式 GB/T 7714 | Andrzejczak, Olga A.,Havelund, Jesper F.,Wang, Wei-Qing,et al. The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(4). |
| APA | Andrzejczak, Olga A..,Havelund, Jesper F..,Wang, Wei-Qing.,Kovalchuk, Sergey.,Hagensen, Christina E..,...&Hebelstrup, Kim H..(2020).The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(4). |
| MLA | Andrzejczak, Olga A.,et al."The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.4(2020). |
入库方式： OAI收割
来源：植物研究所
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