The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development
文献类型：期刊论文
| 作者 | Liu, Jianyang1; Wang, Yanning1; Cheng, Youfa1,2 |
| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2020 |
| 卷号 | 104期号:6页码:1617-1634 |
| ISSN号 | 0960-7412 |
| 关键词 | VPS28 endosomal sorting complex required for transport auxin endosomal sorting embryogenesis vacuole |
| DOI | 10.1111/tpj.15024 |
| 文献子类 | Article |
| 英文摘要 | The highly conserved endosomal sorting complex required for transport (ESCRT) pathway plays critical roles in endosomal sorting of ubiquitinated plasma membrane proteins for degradation. However, the functions of many components of the ESCRT machinery in plants remain unsolved. Here we show that the ESCRT-I subunits VPS28A and VPS28B are functionally redundant and required for embryonic development in Arabidopsis. We conducted a screen for genetic enhancers of pid, which is defective in auxin signaling and transport. We isolated a no--cotyledon in pid 104 (ncp104) mutant, which failed to develop cotyledons in a pid background. We discovered that ncp104 was a unique recessive gain-of-function allele of vps28a. VPS28A and VPS28B were expressed during embryogenesis and the proteins were localized to the trans-Golgi network/early endosome and post-Golgi/endosomal compartments, consistent with their functions in endosomal sorting and embryogenesis. The single vps28a and vps28b loss-of-function mutants did not display obvious developmental defects, but their double mutants showed abnormal cell division patterns and were arrested at the globular embryo stage. The vps28a vps28b double mutants showed altered auxin responses, disrupted PIN1-GFP expression patterns, and abnormal PIN1-GFP accumulation in small aberrant vacuoles. The ncp104 mutation may cause the VPS28A protein to become unstable and/or toxic. Taken together, our findings demonstrate that the ESCRT-I components VPS28A and VPS28B redundantly play essential roles in vacuole formation, endosomal sorting of plasma membrane proteins, and auxin-mediated plant development. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1365-313X |
| 出版地 | HOBOKEN |
| WOS关键词 | PROTEIN-PROTEIN INTERACTIONS ; YUCCA FLAVIN MONOOXYGENASES ; MULTIVESICULAR BODIES ; ARABIDOPSIS ; TRANSPORT ; COMPLEX ; BIOSYNTHESIS ; REVEALS ; LOCALIZATION ; TRAFFICKING |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000587141100001 |
| 资助机构 | National Natural Science Fund of ChinaNational Natural Science Foundation of China (NSFC) [31970309, 31171389, 91217310, 91017008, 31270330] ; National Key Research and Development Program of China [2016YFD0100402] ; National Basic Research Program of ChinaNational Basic Research Program of China [2014CB943400] ; Chinese Academy of Sciences STS Program [KFJ-STS-ZDTP-076] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19101]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jianyang,Wang, Yanning,Cheng, Youfa. The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development[J]. PLANT JOURNAL,2020,104(6):1617-1634. |
| APA | Liu, Jianyang,Wang, Yanning,&Cheng, Youfa.(2020).The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development.PLANT JOURNAL,104(6),1617-1634. |
| MLA | Liu, Jianyang,et al."The ESCRT-I components VPS28A and VPS28B are essential for auxin-mediated plant development".PLANT JOURNAL 104.6(2020):1617-1634. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。