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Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock

文献类型：期刊论文

作者Wang, Yan1; He, Yuqing1; Su, Chen1; Zentella, Rodolfo; Sun, Tai-ping; Wang, Lei1
刊名MOLECULAR PLANT
出版日期2020
卷号13期号:3页码:446-458
ISSN号1674-2052
关键词circadian clock O-fucosyltransferase SPY proteolysis PRR5
DOI10.1016/j.molp.2019.12.013
文献子类Article
英文摘要Post-translational modifications play essential roles in finely modulating eukaryotic circadian clock systems. In plants, the effects of O-glycosylation on the circadian clock and the underlying mechanisms remain largely unknown. The O-fucosyltransferase SPINDLY (SPY) and the O-GlcNAc transferase SECRET AGENT (SEC) are two prominent O-glycosylation enzymes in higher plants, with both overlapped and unique functions in plant growth and development. Unlike the critical role of O-GlcNAc in regulating the animal circadian clock, here we report that nuclear-localized SPY, but not SEC, specifically modulates the pace of the Arabidopsis circadian clock. By identifying the interactome of SPY, we identified PSEUDORESPONSE REGULATOR 5 (PRR5), one of the core circadian clock components, as a new SPY-interacting protein. PRR5 can be O-fucosylated by SPY in planta, while point mutation in the catalytic domain of SPY abolishes the O-fucosylation of PRR5. The protein abundance of PRR5 is strongly increased in spy mutants, while the degradation rate of PRR5 is much reduced, suggesting that PRR5 proteolysis is promoted by SPY-mediated O-fucosylation. Moreover, multiple lines of genetic evidence indicate that PRR5 is a major downstream target of SPY to specifically mediate its modulation of the circadian clock. Collectively, our findings provide novel insights into the specific role of the O-fucosyltransferase activity of SPY in modulating the circadian clock and implicate that O-glycosylation might play an evolutionarily conserved role in modulating the circadian clock system, via O-GlcNAcylation in mammals, but via O-fucosylation in higher plants.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号1752-9867
出版地CAMBRIDGE
WOS关键词PSEUDO-RESPONSE REGULATORS ; GIBBERELLIN-RESPONSE ; SECRET-AGENT ; PHOSPHORYLATION ; REPRESSOR ; PLANT ; GLCNACYLATION ; EXPRESSION ; COMPLEX ; PROTEIN
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
语种英语
出版者CELL PRESS
WOS记录号WOS:000518446000010
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB27030206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31770287] ; National Key Research and Development Program of China [2016YFD0100600] ; National Institutes of HealthUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [R01 GM100051]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19109]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Duke Univ, Dept Biol, Durham, NC 27708 USA
推荐引用方式
GB/T 7714		 Wang, Yan,He, Yuqing,Su, Chen,et al. Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock[J]. MOLECULAR PLANT,2020,13(3):446-458.
APA Wang, Yan,He, Yuqing,Su, Chen,Zentella, Rodolfo,Sun, Tai-ping,&Wang, Lei.(2020).Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock.MOLECULAR PLANT,13(3),446-458.
MLA Wang, Yan,et al."Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock".MOLECULAR PLANT 13.3(2020):446-458.

入库方式： OAI收割

来源：植物研究所

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