Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E
文献类型:期刊论文
作者 | Zhao, Lu1,2; Li, Wenzheng1,2; Wang, Bingwu1,2; Gao, Yulong1,2; Sui, Xueyi1,2; Liu, Yong1,2; Chen, Xuejun1,2; Yao, Xuefeng3,4; Jiao, Fangchan1,2; Song, Zhongbang1,2 |
刊名 | AGRONOMY-BASEL |
出版日期 | 2020 |
卷号 | 10期号:1 |
关键词 | eIF4E1-S potyvirus strains Nicotiana tabacum cv. Yunyan87 EMS-induced nonsense mutation TILLING dCAPS markers |
DOI | 10.3390/agronomy10010036 |
文献子类 | Article |
英文摘要 | Recessive resistance against potyviruses, such as Potato virus Y (PVY), relies on mutations in the eukaryotic translation initiation factor 4E (eIF4E) or one of its isoforms. The eIF4E1-S mutants of burley tobacco (Nicotiana tabacum L.) exhibit recessive resistance against PVY strains. Here, we developed a TILLING population of flue-cured tobacco (N. tabacum cv. Yunyan87) using ethyl methanesulfonate (EMS) to identify eIF4E1-S mutants. M3 plants homozygous for a nonsense mutation in exon 1 of the eIF4E1-S gene demonstrated resistance against PVYMN. These M3 plants were backcrossed to 'Yunyan87', and BC4F3 plants were screened using derived cleaved amplified polymorphic sequence (dCAPS) markers. BC(4)F(3)( )plants showing agronomic traits comparable to the recurrent parent 'Yunyan87' and resistance against PVYO, PVYN, and PVYNTN strains were identified. These genotypes would provide useful germplasm for future tobacco improvement and would aid in basic research on PVY resistance in flue-cured tobacco. |
学科主题 | Agronomy ; Plant Sciences |
电子版国际标准刊号 | 2073-4395 |
出版地 | BASEL |
WOS关键词 | POTATO-VIRUS-Y ; RECESSIVE RESISTANCE ; ARABIDOPSIS-THALIANA ; POTYVIRUS RESISTANCE ; GLANDULAR TRICHOMES ; INITIATION-FACTORS ; NATURAL VARIATION ; GENE ; SUSCEPTIBILITY ; EIF(ISO)4E |
WOS研究方向 | Agriculture ; Plant Sciences |
语种 | 英语 |
出版者 | MDPI |
WOS记录号 | WOS:000513232600036 |
资助机构 | Yunnan Academy of Tobacco Agricultural Sciences [2012YN02, 2017YN02, 2017YN03] |
源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19115] |
专题 | 中科院植物分子生理学重点实验室 |
作者单位 | 1.Yunnan Acad Tobacco Agr Sci, Tobacco Breeding & Biotechnol Res Ctr, Kunming 650021, Yunnan, Peoples R China 2.Key Lab Tobacco Biotechnol Breeding, Kunming 650021, Yunnan, Peoples R China 3.Natl Tobacco Genet Engn Res Ctr, Kunming 650021, Yunnan, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, Lu,Li, Wenzheng,Wang, Bingwu,et al. Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E[J]. AGRONOMY-BASEL,2020,10(1). |
APA | Zhao, Lu.,Li, Wenzheng.,Wang, Bingwu.,Gao, Yulong.,Sui, Xueyi.,...&Song, Zhongbang.(2020).Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E.AGRONOMY-BASEL,10(1). |
MLA | Zhao, Lu,et al."Development of a PVY Resistant Flue-Cured Tobacco Line via EMS Mutagenesis of eIF4E".AGRONOMY-BASEL 10.1(2020). |
入库方式: OAI收割
来源:植物研究所
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