Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum
文献类型：期刊论文
| 作者 | Li, Boqiang; Chen, Yong3; Zong, Yuanyuan; Shang, Yanjiao; Zhang, Zhanquan; Xu, Xiaodi3; Wang, Xiao3; Long, Manyuan2; Tian, Shiping3,4
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| 刊名 | ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 21期号:3页码:1124-1139 |
| ISSN号 | 1462-2912 |
| DOI | 10.1111/1462-2920.14542 |
| 文献子类 | Article |
| 英文摘要 | The patulin biosynthesis is one of model pathways in an understanding of secondary metabolite biology and network novelties in fungi. However, molecular regulation mechanism of patulin biosynthesis and contribution of each gene related to the different catalytic enzymes in the biochemical steps of the pathway remain largely unknown in fungi. In this study, the genetic components of patulin biosynthetic pathway were systematically dissected in Penicillium expansum, which is an important fungal pathogen and patulin producer in harvested fruits and vegetables. Our results revealed that all the 15 genes in the cluster are involved in patulin biosynthesis. Proteins encoded by those genes are compartmentalized in various subcellular locations, including cytosol, nucleus, vacuole, endoplasmic reticulum, plasma membrane and cell wall. The subcellular localizations of some proteins, such as PatE and PatH, are required for the patulin production. Further, the functions of eight enzymes in the 10-step patulin biosynthetic pathway were verified in P. expansum. Moreover, velvet family proteins, VeA, VelB and VelC, were proved to be involved in the regulation of patulin biosynthesis, but not VosA. These findings provide a thorough understanding of the biosynthesis pathway, spatial control and regulation mechanism of patulin in fungi. |
| 学科主题 | Microbiology |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1462-2920 |
| WOS关键词 | SECONDARY METABOLISM ; ASPERGILLUS-NIDULANS ; EXPRESSION ; PATHWAY ; VIRULENCE ; COMPLEX ; PROTEIN ; PATHOGENICITY ; COORDINATION ; MYCOTOXIN |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000461231300018 |
| 出版者 | WILEY |
| 资助机构 | National Key R&D Program of China from Ministry of Science and Technology of the People's Republic of China [2016YFD0400902] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31530057, 31722043] ; Youth Innovation Promotion Association CAS [2015063] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19398]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Minist Educ, Key Lab Biotechnol & Bioresources Utilizat, Dalian 116600, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 4.Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA |
| 推荐引用方式 GB/T 7714 | Li, Boqiang,Chen, Yong,Zong, Yuanyuan,et al. Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum[J]. ENVIRONMENTAL MICROBIOLOGY,2019,21(3):1124-1139. |
| APA | Li, Boqiang.,Chen, Yong.,Zong, Yuanyuan.,Shang, Yanjiao.,Zhang, Zhanquan.,...&Tian, Shiping.(2019).Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum.ENVIRONMENTAL MICROBIOLOGY,21(3),1124-1139. |
| MLA | Li, Boqiang,et al."Dissection of patulin biosynthesis, spatial control and regulation mechanism in Penicillium expansum".ENVIRONMENTAL MICROBIOLOGY 21.3(2019):1124-1139. |
入库方式： OAI收割
来源：植物研究所
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