Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics
文献类型：期刊论文
| 作者 | Li, Huahua; Mei, Xiaoxue; Liu, Bingfeng; Li, Zhen; Wang, Baichen2; Ren, Nanqi; Xing, Defeng |
| 刊名 | ENVIRONMENT INTERNATIONAL
 |
| 出版日期 | 2019 |
| 卷号 | 129页码:1-9 |
| 关键词 | Hydrogen-producing bacteria Ethanoligenens harbinense Acetate-ethanol fermentation Acetic acid tolerance Quantitative proteomics iTRAQ |
| ISSN号 | 0160-4120 |
| DOI | 10.1016/j.envint.2019.05.013 |
| 文献子类 | Article |
| 英文摘要 | Ethanoligenens, a novel ethanologenic hydrogen-producing genus, is a representative fermenter in its unique acetate-ethanol fermentation and physiology. Acetic acid accumulation is one of major factors that affect H-2-ethanol co-production. However, sufficient information is unavailable on the tolerance mechanisms of hydrogen-producing bacterium in acetic acid stress. The fermentation process of Ethanoligenens harbinense YUAN-3 was significantly slowed down in the selection stress of exogenous acetic acid. The maximum gas production rate of strain YUAN-3 decreased from 192.15 mL.(L-culture)(-1).h(-1) to 75.2 mL.(L-culture)(-1).h(-1) with increasing exogenous acetic acid from 0 mM to 30 mM, the batch fermentation period was correspondingly expanded from 66 h to 136 h. Through iTRAQ-based quantitative proteomic approach, 78, 121 and 216 proteins were differentially expressed after strain YUAN-3 was cultured in the medium supplemented with exogenous acetic acid of 10 mM, 20 mM and 30 mM. The up-regulated proteins were mainly involved in beta-alanine and pyrimidine metabolism, oxidative stress response, while the down-regulated proteins mainly participated in phosphotransferase system (PTS), fructose and mannose metabolism, phosphate uptake, ribosome, and flagellar assembly. These proteins help to maintain balance between fermentation process and alleviation of intracellular acidification in strain YUAN-3. The study indicated that response to acetic acid stress in strain YUAN-3 was a complex process, which involved multiple metabolic pathways. Reductive pyrimidine catabolic pathway played an important role in the acetic acid resistance of E. harbinense. |
| 学科主题 | Environmental Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1873-6750 |
| WOS关键词 | BIOHYDROGEN PRODUCTION ; PROTEIN EXPRESSION ; BACTERIA ; REVEALS ; PH ; MECHANISMS ; TOLERANCE ; ENZYMES |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:000470239200001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870114] ; National Key Research and Development Program of China [2018YFD1100503] ; State Key Laboratory of Urban Water Resource and Environment (Harbin Institute of Technology)Harbin Institute of Technology [HCK201018] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19424]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Photosynth Res Ctr, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China 2.Harbin Inst Technol, Sch Environm, State Key Lab Urban Water Resource & Environm, Harbin 150090, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Huahua,Mei, Xiaoxue,Liu, Bingfeng,et al. Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics[J]. ENVIRONMENT INTERNATIONAL,2019,129:1-9. |
| APA | Li, Huahua.,Mei, Xiaoxue.,Liu, Bingfeng.,Li, Zhen.,Wang, Baichen.,...&Xing, Defeng.(2019).Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics.ENVIRONMENT INTERNATIONAL,129,1-9. |
| MLA | Li, Huahua,et al."Insights on acetate-ethanol fermentation by hydrogen-producing Ethanoligenens under acetic acid accumulation based on quantitative proteomics".ENVIRONMENT INTERNATIONAL 129(2019):1-9. |
入库方式： OAI收割
来源：植物研究所
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