Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation
文献类型：期刊论文
| 作者 | Chen, Mei; Liu, Peng3; Zhang, Fin3; Peng, Lianwei2; Huang, Fang |
| 刊名 | INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
 |
| 出版日期 | 2019 |
| 卷号 | 44期号:60页码:31790-31799 |
| ISSN号 | 0360-3199 |
| 关键词 | C. reinhardtii Hydrogen production mutant 91 Sulfur deprivation PSI PSII ATP synthase activity |
| DOI | 10.1016/j.ijhydene.2019.10.074 |
| 文献子类 | Article |
| 英文摘要 | Renewable H-2 photoproduction by Chlamydomonas reinhardtii offers a desirable bio-system for solar fuels. However, its large-scale application is hindered mainly due to lack of ideal strains. We previously isolated a mutant hpm91 which lacks PGR5 and sustains H-2 photoproduction for 25 days. To understand the photosynthetic basis for this remarkable phenotype, we hereby investigated its photochemical characteristics during sulfur-deprived H-2 photoproduction using in vivo chlorophyll fluorescence spectroscopy. Compared to wild type, effective quantum yield of PSII and PSI of hpm91 increased upto 78.9% and 147.6%, respectively. Electron transport rate of each photosystem is closely correlated with the increase of quantum yield, suggesting overall enhanced photochemistry of hpm91 under such condition. Moreover, ATP synthase activity decays slower and remains higher in this mutant. These are in vivo evidence demonstrating increased photosynthetic efficiency of hpm91 promotes its H2 photoproduction. Together with its competent photoheterotrophic growth in a larger photobioreactor, we propose that hpm91 is a valuable strain for re-engineering Chlamydomonas towards improving light energy efficiency in a large-scale system. (C) 2019 Institute of Botany, Chinese Academy of Sciences. Published by Elsevier Ltd on behalf of Hydrogen Energy Publications LLC. |
| 学科主题 | Chemistry, Physical ; Electrochemistry ; Energy & Fuels |
| 电子版国际标准刊号 | 1879-3487 |
| 出版地 | OXFORD |
| WOS关键词 | CYCLIC ELECTRON FLOW ; HYDROGEN-PRODUCTION ; PHOTOSYSTEM-II ; CHLOROPHYLL FLUORESCENCE ; THYLAKOID MEMBRANE ; GREEN-ALGAE ; HIGH-LIGHT ; REINHARDTII ; PROTEIN ; TRANSPORT |
| WOS研究方向 | Chemistry ; Electrochemistry ; Energy & Fuels |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000502888800009 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470340] ; Ministry of Science and Technology of ChinaMinistry of Science and Technology, China [2015CB150100] ; CASChinese Academy of Sciences [17030300, KGCX2-YW-373] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19433]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Mei,Liu, Peng,Zhang, Fin,et al. Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation[J]. INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,2019,44(60):31790-31799. |
| APA | Chen, Mei,Liu, Peng,Zhang, Fin,Peng, Lianwei,&Huang, Fang.(2019).Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation.INTERNATIONAL JOURNAL OF HYDROGEN ENERGY,44(60),31790-31799. |
| MLA | Chen, Mei,et al."Photochemical characteristics of Chlamydomonas mutant hpm91 lacking proton gradient regulation 5 (PGR5) during sustained H-2 photoproduction under sulfur deprivation".INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 44.60(2019):31790-31799. |
入库方式： OAI收割
来源：植物研究所
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