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mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ

文献类型：期刊论文

作者Ding, Shunhua; Zhang, Yi2; Hu, Zhi2; Huang, Xiahe4; Zhang, Bohan2; Lu, Qingtao; Wen, Xiaogang; Wang, Yingchun2,4; Lu, Congming3
刊名MOLECULAR PLANT
出版日期2019
卷号12期号:9页码:1259-1277
关键词mTERF5 transcriptional pausing transcription chloroplast psbEFLJ
ISSN号1674-2052
DOI10.1016/j.molp.2019.05.007
文献子类Article
英文摘要RNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photo-system II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
出版地CAMBRIDGE
电子版国际标准刊号1752-9867
WOS关键词NEGATIVE ELONGATION-FACTOR ; DOMAIN PROTEIN FUNCTIONS ; ENCODED RNA-POLYMERASE ; GENE-EXPRESSION ; PHOTOSYSTEM-I ; COMPLEX REVEALS ; ARABIDOPSIS ; TERMINATION ; PROMOTER ; DNA
WOS研究方向Biochemistry & Molecular Biology ; Plant Sciences
语种英语
WOS记录号WOS:000483463400010
出版者CELL PRESS
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31730102] ; State Key Basic Research and Development Plan of ChinaNational Basic Research Program of China [2015CB150105] ; Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDB17030100] ; National Key Scientific Instrument and Equipment Development Project of China [2013YQ030595]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19457]  
专题中科院光生物学重点实验室
作者单位1.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
3.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Shunhua,Zhang, Yi,Hu, Zhi,et al. mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ[J]. MOLECULAR PLANT,2019,12(9):1259-1277.
APA Ding, Shunhua.,Zhang, Yi.,Hu, Zhi.,Huang, Xiahe.,Zhang, Bohan.,...&Lu, Congming.(2019).mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ.MOLECULAR PLANT,12(9),1259-1277.
MLA Ding, Shunhua,et al."mTERF5 Acts as a Transcriptional Pausing Factor to Positively Regulate Transcription of Chloroplast psbEFLJ".MOLECULAR PLANT 12.9(2019):1259-1277.

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来源：植物研究所

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