SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola
文献类型：期刊论文
| 作者 | Zhao, Haixia2; Wang, Liangsheng ; Zhao, Fang-Jie; Wu, Longhua3; Liu, Anna; Xu, Wenzhong
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| 刊名 | PLANT CELL AND ENVIRONMENT
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| 出版日期 | 2019 |
| 卷号 | 42期号:4页码:1112-1124 |
| 关键词 | cadmium (Cd) chloroplast CRISPR/Cas9 heavy metal ATPase 1 (HMA1) hyperaccumulator Sedum plumbizincicola transporter |
| ISSN号 | 0140-7791 |
| DOI | 10.1111/pce.13456 |
| 文献子类 | Article |
| 英文摘要 | Sedum plumbizincicola is able to hyperaccumulate cadmium (Cd), a nonessential and highly toxic metal, in the above-ground tissues, but the mechanisms for its Cd hypertolerance are not fully understood. Here, we show that the heavy metal ATPase 1 (SpHMA1) of S. plumbizincicola plays an important role in chloroplast Cd detoxification. Compared with the HMA1 ortholog in the Cd nonhyperaccumulating ecotype of Sedum alfredii, the expression of SpHMA1 in the leaves of S. plumbizincicola was >200 times higher. Heterologous expression of SpHMA1 in Saccharomyces cerevisiae increased Cd sensitivity and Cd transport activity in the yeast cells. The SpHMA1 protein was localized to the chloroplast envelope. SpHMA1 RNA interference transgenic plants and CRISPR/Cas9-induced mutant lines showed significantly increased Cd accumulation in the chloroplasts compared with wild-type plants. Chlorophyll fluorescence imaging analysis revealed that the photosystem II of SpHMA1 knockdown and knockout lines suffered from a much higher degree of Cd toxicity than wild type. Taken together, these results suggest that SpHMA1 functions as a chloroplast Cd exporter and protects photosynthesis by preventing Cd accumulation in the chloroplast in S. plumbizincicola and hyperexpression of SpHMA1 is an important component contributing to Cd hypertolerance in S. plumbizincicola. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-3040 |
| WOS关键词 | P-TYPE ATPASE ; CHLOROPHYLL FLUORESCENCE ; INDUCED INHIBITION ; METAL HOMEOSTASIS ; PHOTOSYSTEM-II ; ARABIDOPSIS ; ZINC ; TOLERANCE ; PHOTOSYNTHESIS ; ACCUMULATION |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000462360300003 |
| 出版者 | WILEY |
| 资助机构 | National Key R&D Program of China [2017YFD0800900] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19530]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Soil Environm & Pollut Remediat, Inst Soil Sci, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China 3.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Jiangsu, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,et al. SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola[J]. PLANT CELL AND ENVIRONMENT,2019,42(4):1112-1124. |
| APA | Zhao, Haixia,Wang, Liangsheng,Zhao, Fang-Jie,Wu, Longhua,Liu, Anna,&Xu, Wenzhong.(2019).SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola.PLANT CELL AND ENVIRONMENT,42(4),1112-1124. |
| MLA | Zhao, Haixia,et al."SpHMA1 is a chloroplast cadmium exporter protecting photochemical reactions in the Cd hyperaccumulator Sedum plumbizincicola".PLANT CELL AND ENVIRONMENT 42.4(2019):1112-1124. |
入库方式： OAI收割
来源：植物研究所
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