Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis
文献类型：期刊论文
| 作者 | Liu, Jin; Sun, Zheng2; Mao, Xuemei; Gerken, Henri1; Wang, Xiaofei; Yang, Wenqiang3
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| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2019 |
| 卷号 | 98期号:6页码:1060-1077 |
| 关键词 | Chromochloris zofingiensis glycerol-3-phosphate acyltransferase oleaginousness multiomics trait improvement triacylglycerol |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.14302 |
| 文献子类 | Article |
| 英文摘要 | Chromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating time-resolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and 'pushing' and 'pulling' carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1365-313X |
| WOS关键词 | MICROALGA CHLORELLA-ZOFINGIENSIS ; PENTOSE-PHOSPHATE PATHWAY ; CHLAMYDOMONAS-REINHARDTII ; DIACYLGLYCEROL ACYLTRANSFERASES ; NITROGEN DEPRIVATION ; LIPID-ACCUMULATION ; ACID ACYLTRANSFERASE ; PHYTOENE DESATURASE ; BIOFUEL PRODUCTION ; OIL SYNTHESIS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000473644100009 |
| 出版者 | WILEY |
| 资助机构 | National Youth Thousand Talents Program ; Peking University CCUS project - BHP Billiton ; Double-First Class Initiative of Peking University |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19544]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Shanghai Ocean Univ, Minist Sci & Technol, Int Res Ctr Marine Biosci, Shanghai 201306, Peoples R China 2.Peking Univ, Coll Engn, Lab Algae Biotechnol & Innovat, Beijing 100871, Peoples R China 3.Arizona State Univ Polytech Campus, Sch Sustainable Engn & Built Environm, Mesa, AZ 85212 USA 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jin,Sun, Zheng,Mao, Xuemei,et al. Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis[J]. PLANT JOURNAL,2019,98(6):1060-1077. |
| APA | Liu, Jin,Sun, Zheng,Mao, Xuemei,Gerken, Henri,Wang, Xiaofei,&Yang, Wenqiang.(2019).Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis.PLANT JOURNAL,98(6),1060-1077. |
| MLA | Liu, Jin,et al."Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis".PLANT JOURNAL 98.6(2019):1060-1077. |
入库方式： OAI收割
来源：植物研究所
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