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Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation

文献类型：期刊论文

作者Chai, Fengmei2,3; Liu, Wenwen2,3; Xiang, Yue3; Meng, Xianbin4; Sun, Xiaoming2; Cheng, Cheng3; Liu, Guotian5; Duan, Lixin1; Xin, Haiping; Li, Shaohua2
刊名HORTICULTURE RESEARCH
出版日期2019
卷号6
ISSN号2052-7276
DOI10.1038/s41438-018-0083-5
文献子类Article
英文摘要Vitis amurensis is a wild Vitis plant that can withstand extreme cold temperatures. However, the accumulation of metabolites during cold acclimation (CA) in V. amurensis remains largely unknown. In this study, plantlets of V amurensis and V vinifera cv. Muscat of Hamburg were treated at 4 degrees C for 24 and 72 h, and changes of metabolites in leaves were detected by gas chromatography coupled with time-of-flight mass spectrometry. Most of the identified metabolites, including carbohydrates, amino acids, and organic acids, accumulated in the two types of grape after CA. Galactinol, raffinose, fructose, mannose, glycine, and ascorbate were continuously induced by cold in V amurensis, but not in Muscat of Hamburg. Twelve metabolites, including isoleucine, valine, proline, 2-oxoglutarate, and putrescine, increased in V amurensis during CA. More galactinol, ascorbate, 2-oxoglutarate, and putrescine, accumulated in V amurensis, but not in Muscat of Hamburg, during CA, which may be responsible for the excellent cold tolerance in V amurensis. The expression levels of the genes encoding (3-amylase (BAMY), galactinol synthase (GoIS), and raffinose synthase (RafS) were evaluated by quantitative reverse transcription-PCR. The expression BAMY (VIT_02s0012 g00170) and RafS (VIT_05s0077 g00840) were primarily responsible for the accumulation of maltose and raffinose, respectively. The accumulation of galactinol was attributed to different members of GoIS in the two grapes. In conclusion, these results show the inherent differences in metabolites between V amurensis and V. vinifera under CA.
学科主题Plant Sciences ; Genetics & Heredity ; Horticulture
出版地LONDON
WOS关键词FREEZING TOLERANCE ; ARABIDOPSIS-THALIANA ; TRANSCRIPTION FACTOR ; INTEGRATED ANALYSIS ; GAS-CHROMATOGRAPHY ; ABIOTIC STRESS ; GENES ; SALT ; ACCESSIONS ; RESPONSES
WOS研究方向Plant Sciences ; Genetics & Heredity ; Agriculture
语种英语
WOS记录号WOS:000454602600008
出版者NATURE PUBLISHING GROUP
资助机构Youth Innovation Promotion Association of CAS [2015281] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31471857, 31672132] ; Science and Technology Service Network Initiative of CAS [KFJ-STS-ZDTP-025] ; Grape Breeding Project of Ningxia [NXNYYZ201502]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19571]  
专题中科院植物分子生理学重点实验室
作者单位1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China
2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan, Hubei, Peoples R China
3.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
6.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Chai, Fengmei,Liu, Wenwen,Xiang, Yue,et al. Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation[J]. HORTICULTURE RESEARCH,2019,6.
APA Chai, Fengmei.,Liu, Wenwen.,Xiang, Yue.,Meng, Xianbin.,Sun, Xiaoming.,...&Li, Shaohua.(2019).Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation.HORTICULTURE RESEARCH,6.
MLA Chai, Fengmei,et al."Comparative metabolic profiling of Vitis amurensis and Vitis vinifera during cold acclimation".HORTICULTURE RESEARCH 6(2019).

入库方式： OAI收割

来源：植物研究所

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