The developmental dynamics of the Populus stem transcriptome
文献类型：期刊论文
| 作者 | Chao, Qing; Gao, Zhi-Fang; Zhang, Dong3; Zhao, Biligen-Gaowa; Dong, Feng-Qin; Fu, Chun-Xiang; Liu, Li-Jun; Wang, Bai-Chen |
| 刊名 | PLANT BIOTECHNOLOGY JOURNAL
 |
| 出版日期 | 2019 |
| 卷号 | 17期号:1页码:206-219 |
| 关键词 | PacBio Iso-Seq stem cell maintenance primary growth secondary growth transcription factor |
| ISSN号 | 1467-7644 |
| DOI | 10.1111/pbi.12958 |
| 文献子类 | Article |
| 英文摘要 | The Populus shoot undergoes primary growth (longitudinal growth) followed by secondary growth (radial growth), which produces biomass that is an important source of energy worldwide. We adopted joint PacBio Iso-Seq and RNA-seq analysis to identify differentially expressed transcripts along a developmental gradient from the shoot apex to the fifth internode of Populus Nanlin895. We obtained 87 150 full-length transcripts, including 2081 new isoforms and 62 058 new alternatively spliced isoforms, most of which were produced by intron retention, that were used to update the Populus annotation. Among these novel isoforms, there are 1187 long non-coding RNAs and 356 fusion genes. Using this annotation, we found 15 838 differentially expressed transcripts along the shoot developmental gradient, of which 1216 were transcription factors (TFs). Only a few of these genes were reported previously. The differential expression of these TFs suggests that they may play important roles in primary and secondary growth. AP2, ARF, YABBY and GRF TFs are highly expressed in the apex, whereas NAC, bZIP, PLATZ and HSF TFs are likely to be important for secondary growth. Overall, our findings provide evidence that long-read sequencing can complement short-read sequencing for cataloguing and quantifying eukaryotic transcripts and increase our understanding of the vital and dynamic process of shoot development. |
| 学科主题 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1467-7652 |
| WOS关键词 | SHOOT APICAL MERISTEM ; LONG NONCODING RNAS ; SECONDARY GROWTH ; WOOD FORMATION ; CELL-DIFFERENTIATION ; ARABIDOPSIS GENE ; DNA METHYLATION ; WALL SYNTHESIS ; PLANT ; ROOT |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000455486500018 |
| 出版者 | WILEY |
| 资助机构 | National High Technology Research and Development Program of ChinaNational High Technology Research and Development Program of China [2013AA102701] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19572]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Shandong Agr Univ, Coll Forestry, Tai An, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Biomarker Technol Corp, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chao, Qing,Gao, Zhi-Fang,Zhang, Dong,et al. The developmental dynamics of the Populus stem transcriptome[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(1):206-219. |
| APA | Chao, Qing.,Gao, Zhi-Fang.,Zhang, Dong.,Zhao, Biligen-Gaowa.,Dong, Feng-Qin.,...&Wang, Bai-Chen.(2019).The developmental dynamics of the Populus stem transcriptome.PLANT BIOTECHNOLOGY JOURNAL,17(1),206-219. |
| MLA | Chao, Qing,et al."The developmental dynamics of the Populus stem transcriptome".PLANT BIOTECHNOLOGY JOURNAL 17.1(2019):206-219. |
入库方式： OAI收割
来源：植物研究所
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