Properties and structure of a low-potential, penta-heme cytochrome c(552) from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum
文献类型：期刊论文
| 作者 | Chen, Jing-Hua; Yu, Long-Jiang ; Boussac, Alain5; Wang-Otomo, Zheng-Yu; Kuang, Tingyun; Shen, Jian-Ren
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| 刊名 | PHOTOSYNTHESIS RESEARCH
 |
| 出版日期 | 2019 |
| 卷号 | 139期号:1-3页码:281-293 |
| 关键词 | Purple sulfur bacteria Electron transfer Cytochrome c Multiheme Crystal structure Thermochromatium tepidum |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-018-0507-y |
| 文献子类 | Article; Proceedings Paper |
| 英文摘要 | The thermophilic purple sulfur bacterium Thermochromatium tepidum possesses four main water-soluble redox proteins involved in the electron transfer behavior. Crystal structures have been reported for three of them: a high potential iron-sulfur protein, cytochrome c, and one of two low-potential cytochrome c(552) (which is a flavocytochrome c) have been determined. In this study, we purified another low-potential cytochrome c(552) (LPC), determined its N-terminal amino acid sequence and the whole gene sequence, characterized it with absorption and electron paramagnetic spectroscopy, and solved its high-resolution crystal structure. This novel cytochrome was found to contain five c-type hemes. The overall fold of LPC consists of two distinct domains, one is the five heme-containing domain and the other one is an Ig-like domain. This provides a representative example for the structures of multiheme cytochromes containing an odd number of hemes, although the structures of multiheme cytochromes with an even number of hemes are frequently seen in the PDB database. Comparison of the sequence and structure of LPC with other proteins in the databases revealed several characteristic features which may be important for its functioning. Based on the results obtained, we discuss the possible intracellular function of this LPC in Tch. tepidum. |
| 学科主题 | Plant Sciences |
| 出版地 | DORDRECHT |
| 电子版国际标准刊号 | 1573-5079 |
| WOS关键词 | C NITRITE REDUCTASE ; ANGSTROM RESOLUTION ; CRYSTAL-STRUCTURE ; SPECTROSCOPY ; PROTEINS ; GENES ; CONSERVATION ; ENVIRONMENT ; SUBUNIT ; C554 |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000458553100022 |
| 出版者 | SPRINGER |
| 资助机构 | National Key R&D Program of China [2017YFA0503700] ; Strategic Priority Research Program of CAS [XDB17000000] ; CAS Key Research Project for Frontier Science [QYZDY-SSW-SMC003] ; JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [JP17H06433] ; China Scholarship Council (CSC)China Scholarship Council ; French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19576]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Okayama Univ, Res Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan 2.Chinese Acad Sci, Photosynth Res Ctr, Key Lab Photobiol, Inst Bot, 20 Nanxincun, Beijing 100093, Peoples R China 3.Ibaraki Univ, Fac Sci, Mito, Ibaraki, Japan 4.CEA Saclay, I2BC, SB2SM, CNRS,UMR 9198, F-91191 Gif Sur Yvette, France 5.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jing-Hua,Yu, Long-Jiang,Boussac, Alain,et al. Properties and structure of a low-potential, penta-heme cytochrome c(552) from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum[J]. PHOTOSYNTHESIS RESEARCH,2019,139(1-3):281-293. |
| APA | Chen, Jing-Hua,Yu, Long-Jiang,Boussac, Alain,Wang-Otomo, Zheng-Yu,Kuang, Tingyun,&Shen, Jian-Ren.(2019).Properties and structure of a low-potential, penta-heme cytochrome c(552) from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum.PHOTOSYNTHESIS RESEARCH,139(1-3),281-293. |
| MLA | Chen, Jing-Hua,et al."Properties and structure of a low-potential, penta-heme cytochrome c(552) from a thermophilic purple sulfur photosynthetic bacterium Thermochromatium tepidum".PHOTOSYNTHESIS RESEARCH 139.1-3(2019):281-293. |
入库方式： OAI收割
来源：植物研究所
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