VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine
文献类型：期刊论文
| 作者 | Jiang, Jinzhu4; Xi, Huifen4; Dai, Zhanwu3 ; Lecourieux, Fatma3; Yuan, Ling2; Liu, Xianju4; Patra, Barunava2; Wei, Yongzan; Li, Shaohua; Wang, Lijun
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| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2019 |
| 卷号 | 70期号:2页码:715-729 |
| 关键词 | Fine-tuning grapevine protein interaction resveratrol VvMYB14 VvWRKY8 |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/ery401 |
| 文献子类 | Article |
| 英文摘要 | Resveratrol (Res) is a stilbenoid, a group of plant phenolic metabolites derived from stilbene that possess activities against pests, pathogens, and abiotic stresses. Only a few species, including grapevine (Vitis), synthesize and accumulate Res. Although stilbene synthases (STSs) have been isolated and characterized in several species, the gene regulatory mechanisms underlying stilbene biosynthesis are still largely unknown. Here, we characterize a grapevine WRKY transcription factor, VvWRKY8, that regulates the Res biosynthetic pathway. Transient and stable overexpression of VvWRKY8 in grapevine results in decreased expression of VvSTS15/21 and VvMYB14, as well as in a reduction of Res accumulation. VvWRKY8 does not bind to or activate the promoters of VvMYB14 and VvSTS15/21; however, it physically interacts with VvMYB14 proteins through their N-terminal domains to prevent them from binding to the VvSTS15/21 promoter. Application of exogenous Res results in the stimulation of VvWRKY8 expression and in a decrease of VvMYB14 and VvSTS15/21 expression in grapevine suspension cells, and in the activation of the VvWRKY8 promoter in tobacco leaves. These results demonstrate that VvWRKY8 represses VvSTS15/21 expression and Res biosynthesis through interaction with VvMYB14. In this context, the VvMYB14-VvSTS15/21-Res-VvWRKY8 regulatory loop may be an important mechanism for the fine-tuning of Res biosynthesis in grapevine. |
| 学科主题 | Plant Sciences |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1460-2431 |
| WOS关键词 | WRKY TRANSCRIPTION FACTOR ; SALT STRESS TOLERANCE ; VITIS-VINIFERA ; ULTRAVIOLET C ; BOTRYTIS-CINEREA ; SALICYLIC-ACID ; ABSCISIC-ACID ; PROTEIN ; EXPRESSION ; REGULATOR |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000459350000028 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31171918, 31672120] ; Grape Breeding Project of Ningxia [NXNYYZ201502] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19670]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA 2.Univ Bordeaux, CNRS, INRA, EGFV,Bordeaux Sci Agro,ISVV, F-33140 Villenave Dornon, France 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, CAS Key Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Jinzhu,Xi, Huifen,Dai, Zhanwu,et al. VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(2):715-729. |
| APA | Jiang, Jinzhu.,Xi, Huifen.,Dai, Zhanwu.,Lecourieux, Fatma.,Yuan, Ling.,...&Wang, Lijun.(2019).VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine.JOURNAL OF EXPERIMENTAL BOTANY,70(2),715-729. |
| MLA | Jiang, Jinzhu,et al."VvWRKY8 represses stilbene synthase genes through direct interaction with VvMYB14 to control resveratrol biosynthesis in grapevine".JOURNAL OF EXPERIMENTAL BOTANY 70.2(2019):715-729. |
入库方式： OAI收割
来源：植物研究所
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