Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis
文献类型：期刊论文
| 作者 | Li, Jialong4; Zhang, Fan1; Li, Yuhong4; Yang, Weicai3,4; Lin, Rongcheng4
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| 刊名 | PLANT AND CELL PHYSIOLOGY
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| 出版日期 | 2019 |
| 卷号 | 60期号:11页码:2436-2448 |
| 关键词 | Cell cycle Gametogenesis Light PPO Tetrapyrrole biosynthesis |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcz135 |
| 文献子类 | Article |
| 英文摘要 | Protoporphyrinogen IX oxidase1 (PPO1) catalyzes the oxidation of protoporphyrinogen IX to form protoporphyrin IX in the plastid tetrapyrrole biosynthesis pathway and is also essential for plastid RNA editing in Arabidopsis thaliana. The Arabidopsis ppo1-1 mutation was previously shown to be seedling lethal; however, in this study, we showed that the heterozygous ppo1-1/+ mutant exhibited reproductive growth defects characterized by reduced silique length and seed set, as well as aborted pollen development. In this mutant, the second mitotic division was blocked during male gametogenesis, whereas female gametogenesis was impaired at the one-nucleate stage. Before perishing at the seedling stage, the homozygous ppo1-1 mutant displayed reduced hypocotyl and root length, increased levels of reactive oxygen species accumulation and elevated cell death, especially under light conditions. Wild-type seedlings treated with acifluorfen, a PPO1 inhibitor, showed similar phenotypes to the ppo1-1 mutants, and both plants possessed a high proportion of 2C nuclei and a low proportion of 8C nuclei compared with the untreated wild type. Genome-wide RNA-seq analysis showed that a number of genes, including cell cycle-related genes, were differentially regulated by PPO1. Consistently, PPO1 was highly expressed in the pollen, anther, pistil and root apical meristem cells actively undergoing cell division. Our study reveals a role for PPO1 involved in the mitotic cell cycle during gametogenesis and seedling development. |
| 学科主题 | Plant Sciences ; Cell Biology |
| 出版地 | OXFORD |
| 电子版国际标准刊号 | 1471-9053 |
| WOS关键词 | GAMETOPHYTE DEVELOPMENT ; OXYGEN ; PROGRESSION ; TRANSITION ; CHECKPOINT ; ORGANELLE ; DIVISION ; MUTATION ; DEFECTS ; REVEALS |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000501735800008 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | National Key Research and Development Program of China [2016YFD0100405] ; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2016ZX08009-003] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31870213] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19691]  |
| 专题 | 中科院光生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Mol Dev Biol, Beijing 100101, Peoples R China 2.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jialong,Zhang, Fan,Li, Yuhong,et al. Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis[J]. PLANT AND CELL PHYSIOLOGY,2019,60(11):2436-2448. |
| APA | Li, Jialong,Zhang, Fan,Li, Yuhong,Yang, Weicai,&Lin, Rongcheng.(2019).Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis.PLANT AND CELL PHYSIOLOGY,60(11),2436-2448. |
| MLA | Li, Jialong,et al."Chloroplast-Localized Protoporphyrinogen IX Oxidase1 Is Involved in the Mitotic Cell Cycle in Arabidopsis".PLANT AND CELL PHYSIOLOGY 60.11(2019):2436-2448. |
入库方式： OAI收割
来源：植物研究所
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