Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells
文献类型：期刊论文
| 作者 | Li, Xin5; Diao, Min3,4; Zhang, Yanan2; Chen, Guanlin1; Huang, Shanjin4; Chen, Naizhi |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2019 |
| 卷号 | 20期号:11 |
| 关键词 | guard cell microfilament angle (GCMA) individual actin filaments different orientations actin dynamics technical advance Arabidopsis |
| DOI | 10.3390/ijms20112753 |
| 文献子类 | Article |
| 英文摘要 | The actin cytoskeleton is involved in regulating stomatal movement, which forms distinct actin arrays within guard cells of stomata with different apertures. How those actin arrays are formed and maintained remains largely unexplored. Elucidation of the dynamic behavior of differently oriented actin filaments in guard cells will enhance our understanding in this regard. Here, we initially developed a program called guard cell microfilament analyzer' (GCMA) that enables the selection of individual actin filaments and analysis of their orientations semiautomatically in guard cells. We next traced the dynamics of individual actin filaments and performed careful quantification in open and closed stomata. We found that de novo nucleation of actin filaments occurs at both dorsal and ventral sides of guard cells from open and closed stomata. Interestingly, most of the nucleated actin filaments elongate radially and longitudinally in open and closed stomata, respectively. Strikingly, radial filaments tend to form bundles whereas longitudinal filaments tend to be removed by severing and depolymerization in open stomata. By contrast, longitudinal filaments tend to form bundles that are severed less frequently in closed stomata. These observations provide insights into the formation and maintenance of distinct actin arrays in guard cells in stomata of different apertures. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1422-0067 |
| 出版地 | BASEL |
| WOS关键词 | STOMATAL MOVEMENT ; MICROTUBULE ORGANIZATION ; ABSCISIC-ACID ; REORGANIZATION ; TURNOVER ; CLOSURE ; FUSION ; GROWTH ; ARRAY |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000472634100147 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31671398] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19696]  |
| 专题 | 中科院北方资源植物重点实验室 |
| 作者单位 | 1.OLYMPUS CHINA CO LTD, Beijing 100027, Peoples R China 2.Shanghai Tech Univ, iHuman Inst, Shanghai 201210, Peoples R China 3.Tsinghua Univ, Sch Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 6.Baidu Online Network Technol Beijing CO LTD, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xin,Diao, Min,Zhang, Yanan,et al. Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(11). |
| APA | Li, Xin,Diao, Min,Zhang, Yanan,Chen, Guanlin,Huang, Shanjin,&Chen, Naizhi.(2019).Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(11). |
| MLA | Li, Xin,et al."Guard Cell Microfilament Analyzer Facilitates the Analysis of the Organization and Dynamics of Actin Filaments in Arabidopsis Guard Cells".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.11(2019). |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。