Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica
文献类型：期刊论文
| 作者 | Li, Q. X.; Wang, J.; Zheng, S.; Yang, N.; Sun, K.; He, C. Y. |
| 刊名 | BIOLOGIA PLANTARUM
 |
| 出版日期 | 2019 |
| 卷号 | 63页码:153-163 |
| 关键词 | gene-specific probe GLOBOSA phytoene desaturase PISTILLATA |
| ISSN号 | 0006-3134 |
| DOI | 10.32615/bp.2019.018 |
| 文献子类 | Article |
| 英文摘要 | Virus-induced gene silencing (VIGS) is a particularly useful tool for functional genomics. In the present study, we attempted to utilize this technology to infer the function of genes from Viola philippica using a tobacco rattle virus (TRV) construct. Firstly, the phytoene desaturase gene from V. philippica (VpPDS) was silenced, and local leaf bleaching was observed but did not exhibit systemic effects, thereby limiting utilization of TRV-mediated gene silencing in the recipient plant. However, we observed systemic gene silencing in Nicotiana benthamiana when the VpPDS sequence was used as a trigger, thereby suggesting that heterologous gene sequences could elicit gene silencing. We then investigated the role of gene PISTILLATA from V. philippica (VpPI) of the B-class MADS-box gene family, which regulates the identity and development of stamens and petals. Using the coding region of VpPI as triggers, we determined the gene silencing efficiency of the corresponding GLOBOSA paralogs in N. benthamiana (NbGLO1 and NbGLO2), and we observed stamen-to-carpel transformation and distorted corollas in N. benthamiana suggesting that VpPI functioned as the NbGLO gene. However, the 3'-untranslated region (3'UTR) of VpPI and each 3'UTR of the NbGLO genes downregulated its own corresponding gene, hardly producing floral homeotic alterations. This work provides a better understanding of gene-specific probe design for gene silencing as well as shows that heterologous sequence-triggered VIGS is an efficient way to investigate functional conservation of orthologous genes. |
| 学科主题 | Plant Sciences |
| 出版地 | PRAHA 6 |
| 电子版国际标准刊号 | 1573-8264 |
| WOS关键词 | TOBACCO RATTLE VIRUS ; EXPRESSION ; ARABIDOPSIS ; PISTILLATA ; EVOLUTION ; VECTORS ; FLOWER ; VIGS |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000466354600020 |
| 出版者 | ACAD SCIENCES CZECH REPUBLIC, INST EXPERIMENTAL BOTANY |
| 资助机构 | NSFCNational Natural Science Foundation of China (NSFC) [31560066] ; CAS/SAFEA International Partner Program for Creative Research Teams of Systematic and Evolution Botany |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19708]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Zheng, S. 2.Sun, K.] Northwest Normal Univ, Life Sci Coll, Lanzhou 730070, Gansu, Peoples R China 3.[He, C. Y.] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Wang, J. 5.[Li, Q. X. 6.Yang, N. |
| 推荐引用方式 GB/T 7714 | Li, Q. X.,Wang, J.,Zheng, S.,et al. Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica[J]. BIOLOGIA PLANTARUM,2019,63:153-163. |
| APA | Li, Q. X.,Wang, J.,Zheng, S.,Yang, N.,Sun, K.,&He, C. Y..(2019).Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica.BIOLOGIA PLANTARUM,63,153-163. |
| MLA | Li, Q. X.,et al."Virus-induced gene silencing in Nicotiana benthamiana triggered by heterologous gene sequences from Viola philippica".BIOLOGIA PLANTARUM 63(2019):153-163. |
入库方式： OAI收割
来源：植物研究所
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