CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber
文献类型：期刊论文
| 作者 | Liu, Mengyu; Zhang, Cunjia; Duan, Lixin2; Luan, Qianqian; Li, Jialin; Yang, Aigang3; Qi, Xiaoquan1; Ren, Zhonghai |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2019 |
| 卷号 | 70期号:1页码:69-84 |
| ISSN号 | 0022-0957 |
| 关键词 | CsMYB60 cucumber epigenetic alteration flavonols fruit spine Mutator-like element proanthocyanidins |
| DOI | 10.1093/jxb/ery336 |
| 文献子类 | Article |
| 英文摘要 | Spine colour is an important fruit quality trait that influences the commercial value of cucumber (Cucumis sativus). However, little is known about the metabolites and the regulatory mechanisms of their biosynthesis in black spine varieties. In this study, we determined that the pigments of black spines are flavonoids, including flavonols and proanthocyanidins (PAs). We identified CsMYB60 as the best candidate for the previously identified B (Black spine) locus. Expression levels of CsMYB60 and the key genes involved in flavonoid biosynthesis were higher in black-spine inbred lines than that in white-spine lines at different developmental stages. The insertion of a Mutator-like element (CsMULE) in the second intron of CsMYB60 decreased its expression in a white-spine line. Transient overexpression assays indicated that CsMYB60 is a key regulatory gene and Cs4CL is a key structural gene in the pigmentation of black spines. In addition, the DNA methylation level in the CsMYB60 promoter was much lower in the black-spine line compared with white-spine line. The CsMULE insert may decrease the expression level of CsMYB60, causing hindered synthesis of flavonols and PAs in cucumber fruit spines. |
| 学科主题 | Plant Sciences |
| 电子版国际标准刊号 | 1460-2431 |
| 出版地 | OXFORD |
| WOS关键词 | MUTATOR-LIKE ELEMENTS ; KAPPA-B ; TRANSPOSON ; GENE ; TRANSCRIPTION ; BIOSYNTHESIS ; MAIZE ; DOMESTICATION ; ARABIDOPSIS ; EXPRESSION |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000459348500008 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31672170, 31222048] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [JQ201309] ; Shandong 'Double Tops' Program [SYL2017YSTD06] ; 'Taishan Scholar' Foundation of the People's Government of Shandong Province [ts20130932] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19716]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Agr Univ, Shandong Collaborat Innovat Ctr Fruit & Vegetable, Key Lab Biol & Genet Improvement Hort Crops Huang, State Key Lab Corp Biol,Minist Agr,Coll Hort Sci, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, 20 Nanxincun, Beijing 100093, Peoples R China 3.Guangzhou Univ Chinese Med, Int Inst Translat Chinese Med, Guangzhou, Guangdong, Peoples R China 4.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Mengyu,Zhang, Cunjia,Duan, Lixin,et al. CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber[J]. JOURNAL OF EXPERIMENTAL BOTANY,2019,70(1):69-84. |
| APA | Liu, Mengyu.,Zhang, Cunjia.,Duan, Lixin.,Luan, Qianqian.,Li, Jialin.,...&Ren, Zhonghai.(2019).CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber.JOURNAL OF EXPERIMENTAL BOTANY,70(1),69-84. |
| MLA | Liu, Mengyu,et al."CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber".JOURNAL OF EXPERIMENTAL BOTANY 70.1(2019):69-84. |
入库方式： OAI收割
来源：植物研究所
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