Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Mizoi, Junya; Kanazawa, Natsumi; Kidokoro, Satoshi; Takahashi, Fuminori3; Qin, Feng1,5; Morimoto, Kyoko4; Shinozaki, Kazuo3; Yamaguchi-Shinozaki, Kazuko |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2019 |
| 卷号 | 294期号:3页码:902-917 |
| 关键词 | plant molecular biology transcription factor protein degradation post-translational modification protein phosphorylation abiotic stress response drought tolerance thermotolerance |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.RA118.002662 |
| 文献子类 | Article |
| 英文摘要 | Plants have evolved complex systems to rapidly respond to severe stress conditions, such as heat, cold, and dehydration. Dehydration-responsive element-binding protein 2A (DREB2A) is a key transcriptional activator that induces many heat- and drought-responsive genes, increases tolerance to both heat and drought stress, and suppresses plant growth in Arabidopsis thaliana. DREB2A expression is induced by stress, but stabilization of the DREB2A protein in response to stress is essential for activating the expression of downstream stress-inducible genes. Under nonstress growth conditions, an integral negative regulatory domain (NRD) destabilizes DREB2A, but the mechanism by which DREB2A is stabilized in response to stress remains unclear. Here, based on bioinformatics, mutational, MS, and biochemical analyses, we report that Ser/Thr residues in the NRD are phosphorylated under nonstress growth conditions and that their phosphorylation decreases in response to heat. Furthermore, we found that this phosphorylation is likely mediated by casein kinase 1 and is essential for the NRD-dependent, proteasomal degradation of DREB2A under nonstress conditions. These observations suggest that inhibition of NRD phosphorylation stabilizes and activates DREB2A in response to heat stress to enhance plant thermotolerance. Our study reveals the molecular basis for the coordination of stress tolerance and plant growth through stress-dependent transcriptional regulation, which may allow the plants to rapidly respond to fluctuating environmental conditions. |
| 学科主题 | Biochemistry & Molecular Biology |
| 出版地 | ROCKVILLE |
| 电子版国际标准刊号 | 1083-351X |
| WOS关键词 | CASEIN KINASE-I ; ABIOTIC STRESS ; PROTEINS FUNCTION ; BINDING DOMAIN ; ABSCISIC-ACID ; MAP KINASE ; E3 LIGASE ; GENE ; DROUGHT ; EXPRESSION |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000458670200016 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 资助机构 | JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [JP21780314, JP15K18652, JP15H05960] ; Program for the Promotion of Basic Research Activities for Innovative Biosciences (BRAIN) of Japan |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19745]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.RIKEN Ctr Sustainable Resource Sci, Gene Discovery Res Grp, Yokohama, Kanagawa 2300045, Japan 2.Univ Oxford, Dept Plant Sci, Plant Chemet Lab, South Parks Rd, Oxford OX1 3RB, England 3.Univ Tokyo, Grad Sch Agr & Life Sci, Lab Plant Mol Physiol, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan 4.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 5.Japan Int Res Ctr Agr Sci, Biol Resources & Postharvest Div, Tsukuba, Ibaraki 3058686, Japan |
| 推荐引用方式 GB/T 7714 | Mizoi, Junya,Kanazawa, Natsumi,Kidokoro, Satoshi,et al. Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2019,294(3):902-917. |
| APA | Mizoi, Junya.,Kanazawa, Natsumi.,Kidokoro, Satoshi.,Takahashi, Fuminori.,Qin, Feng.,...&Yamaguchi-Shinozaki, Kazuko.(2019).Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana.JOURNAL OF BIOLOGICAL CHEMISTRY,294(3),902-917. |
| MLA | Mizoi, Junya,et al."Heat-induced inhibition of phosphorylation of the stress-protective transcription factor DREB2A promotes thermotolerance of Arabidopsis thaliana".JOURNAL OF BIOLOGICAL CHEMISTRY 294.3(2019):902-917. |
入库方式： OAI收割
来源：植物研究所
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