Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes
文献类型：期刊论文
| 作者 | Yang, Qi; Han, Xue-Min; Gu, Jin-Ke; Liu, Yan-Jing; Yang, Mao-Jun; Zeng, Qing-Yin |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2019 |
| 卷号 | 221期号:2页码:1060-1073 |
| 关键词 | functional divergence glutathione S-transferase (GST) orthologous gene Populus protein function |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.15430 |
| 文献子类 | Article |
| 英文摘要 | A common assumption in comparative genomics is that orthologous genes are functionally more similar than paralogous genes. However, the validity of this assumption needs to be assessed using robust experimental data. We conducted tissue-specific gene expression and protein function analyses of orthologous groups within the glutathione S-transferase (GST) gene family in three closely related Populus species: Populus trichocarpa, Populus euphratica and Populus yatungensis. This study identified 21 GST orthologous groups in the three Populus species. Although the sequences of the GST orthologous groups were highly conserved, the divergence in enzymatic functions was prevalent. Through site-directed mutagenesis of orthologous proteins, this study revealed that nonsynonymous substitutions at key amino acid sites played an important role in the divergence of enzymatic functions. In particular, a single amino acid mutation (Arg39 -> Trp39) contributed to P. euphratica PeGSTU30 possessing high enzymatic activity via increasing the hydrophobicity of the active cavity. This study provided experimental evidence showing that orthologues belonging to the gene family have functional divergences. The nonsynonymous substitutions at a few amino acid sites resulted in functional divergence of the orthologous genes. Our findings provide new insights into the evolution of orthologous genes in closely related species. |
| 学科主题 | Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1469-8137 |
| WOS关键词 | GLUTATHIONE-S-TRANSFERASE ; SUPERGENE FAMILY ; DUPLICATE GENES ; EVOLUTIONARY ; GENOME ; DIVERGENCE ; RESISTANCE ; RESIDUES |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000453883200044 |
| 出版者 | WILEY |
| 资助机构 | National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [31425006] ; Chinese Academy of Forestry [CAFYBB2018ZX001] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19751]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Han, Xue-Min 2.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China 3.[Gu, Jin-Ke 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 6.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China 7.Liu, Yan-Jing 8.[Yang, Qi |
| 推荐引用方式 GB/T 7714 | Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,et al. Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes[J]. NEW PHYTOLOGIST,2019,221(2):1060-1073. |
| APA | Yang, Qi,Han, Xue-Min,Gu, Jin-Ke,Liu, Yan-Jing,Yang, Mao-Jun,&Zeng, Qing-Yin.(2019).Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes.NEW PHYTOLOGIST,221(2),1060-1073. |
| MLA | Yang, Qi,et al."Functional and structural profiles of GST gene family from three Populus species reveal the sequence-function decoupling of orthologous genes".NEW PHYTOLOGIST 221.2(2019):1060-1073. |
入库方式： OAI收割
来源：植物研究所
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