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Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus

文献类型：期刊论文

作者Sipka, Gabor; Mueller, Pavel1; Brettel, Klaus1; Magyar, Melinda; Kovacs, Laszlo; Zhu, Qingjun; Xiao, Yanan; Han, Guangye; Lambrev, Petar H.; Shen, Jian-Ren
刊名PHYSIOLOGIA PLANTARUM
出版日期2019
卷号166期号:1页码:22-32
ISSN号0031-9317
DOI10.1111/ppl.12945
文献子类Article; Proceedings Paper
英文摘要Recent chlorophyll-a fluorescence yield measurements, using single-turnover saturating flashes (STSFs), have revealed the involvement of a rate-limiting step in the reactions following the charge separation induced by the first flash. As also shown here, in diuron-inhibited PSII core complexes isolated from Thermosynechococcus vulcanus the fluorescence maximum could only be reached by a train of STSFs. In order to elucidate the origin of the fluorescence yield increments in STSF series, we performed transient absorption measurements at 819nm, reflecting the photooxidation and re-reduction kinetics of the primary electron donor P680. Upon single flash excitation of the dark-adapted sample, the decay kinetics could be described with lifetimes of 17ns (approximate to 50%) and 167ns (approximate to 30%), and a longer-lived component (approximate to 20%). This kinetics are attributed to re-reduction of P680(center dot+) by the donor side of PSII. In contrast, upon second-flash (with t between 5s and 100ms) or repetitive excitation, the 819nm absorption changes decayed with lifetimes of about 2ns (approximate to 60%) and 10ns (approximate to 30%), attributed to recombination of the primary radical pair P680(center dot+)Pheo(center dot-), and a small longer-lived component (approximate to 10%). These data confirm that only the first STSF is capable of generating stable charge separation-leading to the reduction of Q(A); and thus, the fluorescence yield increments elicited by the consecutive flashes must have a different physical origin. Our double-flash experiments indicate that the rate-limiting steps, detected by chlorophyll-a fluorescence, are not correlated with the turnover of P680.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1399-3054
WOS关键词PRIMARY ELECTRON-DONOR ; PRIMARY-CHARGE SEPARATION ; REDUCTION KINETICS ; ABSORPTION-SPECTROSCOPY ; RECOMBINATION PATHWAYS ; ABSORBENCY CHANGES ; P-680 ; PHEOPHYTIN ; EXCITATION ; PARTICLES
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000466108300004
出版者WILEY
资助机构National Research Development and Innovation Office of HungaryNational Research, Development & Innovation Office (NRDIO) - Hungary [NKFIH OTKA KH 124985, K 128679, TET_15-1-2016-0144, NKFIH OTKA NN 124904, GINOP-2.3.2-15-2016-00001, OTKA PD 121225] ; STSM Grant from the EBSA ; French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05] ; National Key R&D Program of China [2017YFA0503700] ; Strategic Priority Research Program of Chinese Academy of SciencesChinese Academy of Sciences [XDB17000000] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31470339] ; Lendulet Program of the Hungarian Academy of Sciences [LP2014/19]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19768]  
专题中科院光生物学重点实验室
作者单位1.Hungarian Acad Sci, Biol Res Ctr, Inst Plant Biol, Lab Photosynthet Membranes, Szeged, Hungary
2.Univ Paris Saclay, Univ Paris Sud, Inst Integrat Biol Cell I2BC, CEA,CNRS, F-91198 Gif Sur Yvette, France
3.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Beijing, Peoples R China
4.Okayama Univ, Photosynth Res Ctr, Okayama, Japan
5.Univ Ostrava, Fac Sci, Ostrava, Czech Republic
推荐引用方式
GB/T 7714		 Sipka, Gabor,Mueller, Pavel,Brettel, Klaus,et al. Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus[J]. PHYSIOLOGIA PLANTARUM,2019,166(1):22-32.
APA Sipka, Gabor.,Mueller, Pavel.,Brettel, Klaus.,Magyar, Melinda.,Kovacs, Laszlo.,...&Garab, Gyozo.(2019).Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus.PHYSIOLOGIA PLANTARUM,166(1),22-32.
MLA Sipka, Gabor,et al."Redox transients of P680 associated with the incremental chlorophyll-a fluorescence yield rises elicited by a series of saturating flashes in diuron-treated photosystem II core complex of Thermosynechococcus vulcanus".PHYSIOLOGIA PLANTARUM 166.1(2019):22-32.

入库方式： OAI收割

来源：植物研究所

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