DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae
文献类型：期刊论文
| 作者 | Ren, Feng-Ming; Wang, Ying-Wei; Xu, Zhi-Chao; Li, Ying; Xin, Tian-Yi; Zhou, Jian-Guo; Qi, Yao-Dong; Wei, Xue-Ping; Yao, Hui; Song, Jing-Yuan |
| 刊名 | ECOLOGY AND EVOLUTION
 |
| 出版日期 | 2019 |
| 卷号 | 9期号:4页码:1934-1945 |
| 关键词 | biodiversity hotspot Corydalis DNA barcoding matK species identification the internal transcribed spacer |
| ISSN号 | 2045-7758 |
| DOI | 10.1002/ece3.4886 |
| 文献子类 | Article |
| 英文摘要 | The genus Corydalis is recognized as one of the most taxonomically challenging plant taxa. It is mainly distributed in the Himalaya-Hengduan Mountains, a global biodiversity hotspot. To date, no effective solution for species discrimination and taxonomic assignment in Corydalis has been developed. In this study, five nuclear and chloroplast DNA regions, ITS, ITS2, matK, rbcL, and psbA-trnH, were preliminarily assessed based on their ability to discriminate Corydalis to eliminate inefficient regions, and the three regions showing good performance (ITS, ITS2 and matK) were then evaluated in 131 samples representing 28 species of 11 sections of four subgenera in Corydalis using three analytical methods (NJ, ML, MP tree; K2P-distance and BLAST). The results showed that the various approaches exhibit different species identification power and that BLAST shows the best performance among the tested approaches. A comparison of different barcodes indicated that among the single barcodes, ITS (65.2%) exhibited the highest identification success rate and that the combination of ITS+matK (69.6%) provided the highest species resolution among all single barcodes and their combinations. Three Pharmacopoeia-recorded medicinal plants and their materia medica were identified successfully based on the ITS and ITS2 regions. In the phylogenetic analysis, the sections Thalictrifoliae, Sophorocapnos, Racemosae, Aulacostigma, and Corydalis formed well-supported separate lineages. We thus hypothesize that the five sections should be classified as an independent subgenus and that the genus should be divided into three subgenera. In this study, DNA barcoding provided relatively high species discrimination power, indicating that it can be used for species discrimination in this taxonomically complicated genus and as a potential tool for the authentication of materia medica belonging to Corydalis. |
| 学科主题 | Ecology ; Evolutionary Biology |
| 出版地 | HOBOKEN |
| WOS关键词 | IDENTIFICATION ; BIODIVERSITY ; AUTHENTICATION ; DIVERSITY ; RHIZOMA ; UTILITY ; L. |
| WOS研究方向 | Environmental Sciences & Ecology ; Evolutionary Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000461114900031 |
| 出版者 | WILEY |
| 资助机构 | Basic scientific research project of Chongqing-18 [2018CSCT-JXJL-JBKY-0034] ; CAMS Innovation Fund for Medical Sciences (CIFMS) [2017-I2M-1-009] ; Science and technology project of traditional Chinese medicine in Chongqing [ZY201702143] ; Basic scientific research project of Chongqing-16 [2016CSTC -JBKY01308] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/19780]  |
| 专题 | 植物园 |
| 作者单位 | 1.Zhou, Jian-Guo 2.Qi, Yao-Dong 3.Wei, Xue-Ping 4.Yao, Hui 5.Chinese Acad Med Sci, Inst Med Plant Dev, Peking Union Med Coll,Key Lab Chinese Med Resourc, State Adm Tradit Chinese Med Peoples Republ China, Beijing, Peoples R China 6.Chinese Med Breeding & Evaluat Engn Technol Res C, Characterist Biol Resources Sichuan & Chongqing C, Chongqing Inst Med Plant Cultivat Res & Utilizat, Chongqing, Peoples R China 7.Chinese Acad Sci, Inst Bot, Beijing Bot Garden, Beijing, Peoples R China 8.[Ren, Feng-Ming 9.Xu, Zhi-Chao 10.Li, Ying |
| 推荐引用方式 GB/T 7714 | Ren, Feng-Ming,Wang, Ying-Wei,Xu, Zhi-Chao,et al. DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae[J]. ECOLOGY AND EVOLUTION,2019,9(4):1934-1945. |
| APA | Ren, Feng-Ming.,Wang, Ying-Wei.,Xu, Zhi-Chao.,Li, Ying.,Xin, Tian-Yi.,...&Song, Jing-Yuan.(2019).DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae.ECOLOGY AND EVOLUTION,9(4),1934-1945. |
| MLA | Ren, Feng-Ming,et al."DNA barcoding of Corydalis, the most taxonomically complicated genus of Papaveraceae".ECOLOGY AND EVOLUTION 9.4(2019):1934-1945. |
入库方式： OAI收割
来源：植物研究所
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