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Structure of a C2S2M2N2 type PSII-LHCII supercomplex from the green alga Chlamydomonas reinhardtii

文献类型：期刊论文

作者Shen, Liangliang1; Huang, Zihui2,3,8; Chang, Shenghai2,3,8; Wang, Wenda; Wang, Jingfen4; Kuang, Tingyun; Han, Guangye; Shen, Jian-Ren; Zhang, Xing2,3,4,8
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2019
卷号116期号:42页码:21246-21255
关键词photosynthesis cryoelectron microscopy photosystem II light-harvesting complex structural analysis
ISSN号0027-8424
DOI10.1073/pnas.1912462116
文献子类Article
英文摘要Photosystem II (PSII) in the thylakoid membranes of plants, algae, and cyanobacteria catalyzes light-induced oxidation of water by which light energy is converted to chemical energy and molecular oxygen is produced. In higher plants and most eukaryotic algae, the PSII core is surrounded by variable numbers of light-harvesting antenna complex II (LHCII), forming a PSII-LHCII supercomplex. In order to harvest energy efficiently at low-light-intensity conditions under water, a complete PSII-LHCII supercomplex (C2S2M2N2) of the green alga Chlamydomonas reinhardtii (Cr) contains more antenna subunits and pigments than the dominant PSII-LHCII supercomplex (C2S2M2) of plants. The detailed structure and energy transfer pathway of the Cr-PSII-LHCII remain unknown. Here we report a cryoelectron microscopy structure of a complete, C2S2M2N2-type PSIILHCII supercomplex from C. reinhardtii at 3.37-A resolution. The results show that the Cr-C2S2M2N2 supercomplex is organized as a dimer, with 3 LHCII trimers, 1 CP26, and 1 CP29 peripheral antenna subunits surrounding each PSII core. The N-LHCII trimer partially occupies the position of CP24, which is present in the higher-plant PSII-LHCII but absent in the green alga. The M trimer is rotated relative to the corresponding M trimer in plant PSII-LHCII. In addition, some unique features were found in the green algal PSII core. The arrangement of a huge number of pigments allowed us to deduce possible energy transfer pathways from the peripheral antennae to the PSII core.
学科主题Multidisciplinary Sciences
出版地WASHINGTON
WOS关键词LIGHT-HARVESTING COMPLEX ; ENERGY-TRANSFER PATHWAYS ; EVOLVING PHOTOSYSTEM-II ; CRYSTAL-STRUCTURE ; SUPRAMOLECULAR ORGANIZATION ; RESOLUTION ; ANTENNA ; POLYPEPTIDES ; ARCHITECTURE ; ARRANGEMENT
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000490183000064
出版者NATL ACAD SCIENCES
资助机构National Key R&D Program of China [2017YFA0503700, 2017YFA0504803, 2018YFA0507700] ; Chinese Academy of Sciences Key Research Program of Frontier Sciences [QYZDY-SSW-SMC003] ; Strategic Priority Research Program of CAS [XDB17000000] ; National Basic Research Program of ChinaNational Basic Research Program of China [2015CB150100] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2018XZZX001-13]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19795]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Photosynthesis Res Ctr, Key Lab Photobiol, Beijing 100093, Peoples R China
2.Zhejiang Univ, Sch Med, Dept Biophys, Hangzhou 310058, Zhejiang, Peoples R China
3.Zhejiang Univ, Sch Med, Dept Pathol, Sir Run Run Shaw Hosp, Hangzhou 310058, Zhejiang, Peoples R China
4.Zhejiang Univ, Sch Med, Ctr Cryo Electron Microscopy, Hangzhou 310058, Zhejiang, Peoples R China
5.Zhejiang Univ, Sch Med, Dept Cell Biol, Hangzhou 310058, Zhejiang, Peoples R China
6.Okayama Univ, Inst Interdisciplinary Sci, Okayama 7008530, Japan
7.Okayama Univ, Grad Sch Nat Sci & Technol, Okayama 7008530, Japan
8.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Shen, Liangliang,Huang, Zihui,Chang, Shenghai,et al. Structure of a C2S2M2N2 type PSII-LHCII supercomplex from the green alga Chlamydomonas reinhardtii[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(42):21246-21255.
APA Shen, Liangliang.,Huang, Zihui.,Chang, Shenghai.,Wang, Wenda.,Wang, Jingfen.,...&Zhang, Xing.(2019).Structure of a C2S2M2N2 type PSII-LHCII supercomplex from the green alga Chlamydomonas reinhardtii.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(42),21246-21255.
MLA Shen, Liangliang,et al."Structure of a C2S2M2N2 type PSII-LHCII supercomplex from the green alga Chlamydomonas reinhardtii".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.42(2019):21246-21255.

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来源：植物研究所

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