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The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance

文献类型：期刊论文

作者Sun, Xiaoming3; Zhang, Langlang; Wong, Darren C. J.; Wang, Yi3; Zhu, Zhenfei; Xu, Guangzhao3; Wang, Qingfeng; Li, Shaohua3; Liang, Zhenchang3; Xin, Haiping
刊名PLANT JOURNAL
出版日期2019
卷号99期号:5页码:988-1002
关键词cold stress ERF transcription factor WRKY transcription factor GCC-box Vitis amurensis
ISSN号0960-7412
DOI10.1111/tpj.14378
文献子类Article
英文摘要Cold stress is a major limiting factor in grape (Vitis) productivity. In this study, we characterized a cold-responsive ethylene response factor (ERF) transcription factor, VaERF092, from Amur grape (Vitis amurensis). VaERF092 expression was induced by both low temperatures and the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC), but was suppressed by treatment with the ethylene inhibitor aminoethoxyvinylglycine (AVG) under cold conditions. Ectopic expression of VaERF092 in Arabidopsis thaliana enhanced cold tolerance. Co-expression network analysis of V. vinifera genes indicated that WRKY33 might be a downstream target of VaERF092. This hypothesis was supported by the fact that VaWRKY33 was expressed temporally after VaERF092 expression and could also be induced by cold and ACC, and inhibited by AVG. Yeast one-hybrid, transient beta-glucuronidase (GUS) and dual-luciferase reporter assays provided evidence for an interaction between VaERF092 and a GCC-box element in the VaWRKY33 promoter. In addition, heterologous overexpression of VaWRKY33 in A. thaliana resulted in enhanced cold tolerance. VaERF092- and VaWRKY33 overexpressing grape calli showed lower low-temperature exothermic values than the empty vector (EV) calli, indicating enhanced tolerance to cold. Together, these results indicated that VaERF092 regulates VaWRKY33 through binding to its promoter GCC-box, leading to enhanced cold stress tolerance.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1365-313X
WOS关键词ABIOTIC STRESS TOLERANCE ; GENOME-WIDE ANALYSIS ; INDUCED CHILLING TOLERANCE ; ERF GENE FAMILY ; FREEZING TOLERANCE ; ENHANCES RESISTANCE ; VITIS-AMURENSIS ; LOW-TEMPERATURE ; OSMOTIC-STRESS ; ARABIDOPSIS
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000483586500014
出版者WILEY
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/19807]  
专题中科院北方资源植物重点实验室
作者单位1.Wong, Darren C. J.] Australian Natl Univ, Res Sch Biol, Ecol & Evolut, Acton, ACT 2601, Australia
2.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, CAS Key Lab Plant Resources, Beijing 100093, Peoples R China
3.Chinese Acad Sci, Key Lab Plant Germ Plasm Enhancement & Specialty, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Xiaoming,Zhang, Langlang,Wong, Darren C. J.,et al. The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance[J]. PLANT JOURNAL,2019,99(5):988-1002.
APA Sun, Xiaoming.,Zhang, Langlang.,Wong, Darren C. J..,Wang, Yi.,Zhu, Zhenfei.,...&Xin, Haiping.(2019).The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance.PLANT JOURNAL,99(5),988-1002.
MLA Sun, Xiaoming,et al."The ethylene response factor VaERF092 from Amur grape regulates the transcription factor VaWRKY33, improving cold tolerance".PLANT JOURNAL 99.5(2019):988-1002.

入库方式： OAI收割

来源：植物研究所

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